US 12,465,047 B2
Preparation method for L-glufosinate-ammonium powder
Yaping Xue, Hangzhou (CN); Jie Mao, Hangzhou (CN); Feng Cheng, Hangzhou (CN); Shuping Zou, Hangzhou (CN); Jianmiao Xu, Hangzhou (CN); and Yuguo Zheng, Hangzhou (CN)
Assigned to ZHEJIANG UNIVERSITY OF TECHNOLOGY, Hangzhou (CN)
Appl. No. 17/927,867
Filed by ZHEJIANG UNIVERSITY OF TECHNOLOGY, Hangzhou (CN)
PCT Filed Dec. 26, 2020, PCT No. PCT/CN2020/139769
§ 371(c)(1), (2) Date Nov. 25, 2022,
PCT Pub. No. WO2022/041603, PCT Pub. Date Mar. 3, 2022.
Claims priority of application No. 202010873717.2 (CN), filed on Aug. 26, 2020.
Prior Publication US 2023/0225314 A1, Jul. 20, 2023
Int. Cl. A01N 25/12 (2006.01); A01N 57/20 (2006.01)
CPC A01N 25/12 (2013.01) [A01N 57/20 (2013.01)] 9 Claims
 
1. A method for preparing L-glufosinate ammonium powder, comprising the following steps:
(1) obtaining a transformation solution for performing biocatalytic transformation to prepare L-glufosinate ammonium, and filtering out bacteria to obtain a filtrate;
(2) detecting the amount of ammonium sulfate in the filtrate obtained in step (1), adding calcium hydroxide or calcium oxide to react with the ammonium sulfate to produce calcium sulfate precipitates, and filtering out the calcium sulfate precipitates to obtain a filtrate;
(3) detecting the amount of glufosinate ammonium in the filtrate obtained in step (2), adding zinc salt, adjusting pH to 5.5-6.8 to produce glufosinate ammonium zinc salt precipitates, and filtering and collecting the glufosinate ammonium zinc salt precipitates,
wherein the zinc salt is zinc nitrate or zinc chloride;
(4) adding a solvent to dissolve the glufosinate ammonium zinc salt precipitates collected in step (3), and adjusting pH of a solution to 2-2.5 to produce L-glufosinate ammonium precipitates; and
(5) recrystallizing the L-glufosinate ammonium precipitates obtained in step (4) to obtain purified L-glufosinate ammonium powder.