US 12,135,413 B2
System and method for microscope high-speed auto-focusing including an imaged subject based on corrected contrast distribution
Shiou-jyh Ja, Portland, OR (US)
Assigned to ARACELI BIOSCIENCES INC., Tigard, OR (US)
Filed by ARACELI BIOSCIENCES INC., Tigard, OR (US)
Filed on Jun. 7, 2023, as Appl. No. 18/331,077.
Application 18/331,077 is a continuation of application No. 17/808,491, filed on Jun. 23, 2022, granted, now 11,714,273.
Prior Publication US 2023/0418040 A1, Dec. 28, 2023
Int. Cl. G02B 21/24 (2006.01); G02B 21/36 (2006.01)
CPC G02B 21/244 (2013.01) [G02B 21/367 (2013.01)] 20 Claims
OG exemplary drawing
 
1. A method for auto-focusing a microscope, comprising:
measuring a first focus of the microscope relative to a subject in an initial position, including performing a first direct measurement of the first focus from image content acquired with the microscope while the subject is in the initial position;
moving the subject by a first amount from the initial position to a second position, and measuring a second focus of the microscope relative to the subject in the second position, including performing a second direct measurement of the second focus from image content acquired with the microscope while the subject is in the second position;
moving the subject by a second amount from the second position to a third position, where the third position is based on a difference between the measured first focus and the measured second focus, and measuring a third focus of the microscope relative to the subject in the third position, including performing a third direct measurement of the third focus from image content acquired with the microscope while the subject is in the third position; and
moving the subject by a third amount from the third position to a fourth position, where the fourth position is determined via a modeled curve including the measured first focus, the measured second focus, and the measured third focus.