	US 12,135,333 B2
	Detection kit for detecting immunosuppressors in whole blood by high performance liquid chromatography-tandem mass spectrometry and detection method thereof
Shanshan Zeng, Hangzhou (CN); Qi Zhang, Hangzhou (CN); Baoyu Han, Hangzhou (CN); and Huafen Liu, Hangzhou (CN)
Assigned to HANGZHOU CALIBRA DIAGNOSTICS CO., LTD., Hangzhou (CN)
Filed by Hangzhou Calibra Diagnostics Co., Ltd., Zhejiang (CN)
Filed on Oct. 27, 2021, as Appl. No. 17/511,861.
Application 17/511,861 is a continuation of application No. 17/460,739, filed on Aug. 30, 2021, abandoned.
Claims priority of application No. 202110673324.1 (CN), filed on Jun. 17, 2021.
Prior Publication US 2022/0404380 A1, Dec. 22, 2022
Int. Cl. G01N 33/96 (2006.01); G01N 1/28 (2006.01); G01N 1/38 (2006.01); G01N 30/02 (2006.01); G01N 30/04 (2006.01); G01N 30/06 (2006.01); G01N 30/34 (2006.01); G01N 30/72 (2006.01); G01N 30/88 (2006.01); G01N 33/68 (2006.01); G01N 33/94 (2006.01)

CPC G01N 33/96 (2013.01) [G01N 1/286 (2013.01); G01N 1/38 (2013.01); G01N 30/06 (2013.01); G01N 30/34 (2013.01); G01N 30/7233 (2013.01); G01N 33/9493 (2013.01); G01N 2030/027 (2013.01); G01N 2030/045 (2013.01); G01N 2030/8822 (2013.01); G01N 2030/8831 (2013.01); G01N 33/6848 (2013.01)]

18 Claims

1. A method for detecting an immunosuppressor in whole blood, comprising the steps:

providing a detection kit comprising:

an internal standard solution, wherein the internal standard solution comprises an additive, and the additive is one or more of 2,6-di-tert-butyl-p-cresol, vitamin E, vitamin C, β-carotene, and sodium metabisulfite;

performing a system suitability test, preparing a sample, pre-treating the sample and detecting the sample; wherein the sample pretreatment comprises the following steps:

taking the internal standard solution and internal standard diluent from the detection kit for mixing according to a ratio of 1:24 to obtain an internal standard working solution;

taking a sample and the internal standard working solution for mixing evenly according to a ratio of 1:3-1:7, and performing centrifugation, then taking supernatant for detection by high performance liquid chromatography-tandem mass spectrometry.