	US 12,135,332 B2
	Mutant human estrogen receptor-α and methods of use thereof
Sichun Yang, Solon, OH (US); and Yi Peng, Cleveland, OH (US)
Assigned to CASE WESTERN RESERVE UNIVERSITY, Cleveland, OH (US)
Filed by Case Western Reserve University, Cleveland, OH (US)
Filed on Nov. 26, 2019, as Appl. No. 16/695,487.
Claims priority of provisional application 62/893,856, filed on Aug. 30, 2019.
Claims priority of provisional application 62/772,296, filed on Nov. 28, 2018.
Prior Publication US 2020/0166530 A1, May 28, 2020
Int. Cl. C07K 14/47 (2006.01); C07K 14/525 (2006.01); G01N 33/566 (2006.01); G01N 33/574 (2006.01); G01N 33/94 (2006.01)

CPC G01N 33/94 (2013.01) [C07K 14/4748 (2013.01); C07K 14/525 (2013.01); G01N 33/566 (2013.01); G01N 33/5748 (2013.01); G01N 2500/04 (2013.01); G01N 2500/20 (2013.01)]

10 Claims

1. An isolated mutant human estrogen receptor alpha (hERα) comprising a DNA-binding domain (DBD), a ligand-binding domain (LBD), and an interface between the DBD and the LBD, wherein the tryptophan residue W200 is not mutated to a phenylalanine residue, and wherein at least three tryptophan residues selected from W292, W360, W383, and W393 are mutated to a phenylalanine residue.