	US 12,135,330 B2
	Articles and methods involving detection of binding
Michael D. Scholle, Bolingbrook, IL (US); and John Sherrill, San Diego, CA (US)
Assigned to Charles River Laboratories International, Inc., Wilmington, MA (US)
Appl. No. 16/497,485
Filed by Charles River Laboratories International, Inc., Wilmington, MA (US)
PCT Filed Mar. 28, 2018, PCT No. PCT/US2018/024821 § 371(c)(1), (2) Date Sep. 25, 2019, PCT Pub. No. WO2018/183482, PCT Pub. Date Oct. 4, 2018.
Claims priority of provisional application 62/480,161, filed on Mar. 31, 2017.
Prior Publication US 2020/0018769 A1, Jan. 16, 2020
Int. Cl. G01N 33/68 (2006.01); C40B 30/04 (2006.01); H01J 49/16 (2006.01); H01J 49/04 (2006.01)

CPC G01N 33/6851 (2013.01) [C40B 30/04 (2013.01); H01J 49/164 (2013.01); G01N 2610/00 (2013.01); H01J 49/0418 (2013.01)]

20 Claims

1. A method for detecting binding between a first species and a second species, comprising:

exposing the first species to a composition comprising the second species;

capturing the first species on a self-assembled monolayer, wherein the self-assembled monolayer comprises a standard species prior to the capture of the first species thereon;

performing MALDI on the self-assembled monolayer to generate a signal from the second species and a signal from the standard species;

measuring the signal from the second species;

measuring the signal from the standard species; and

determining whether binding has occurred if a ratio of the signal from the second species to the signal from the standard species is greater than or equal to a cutoff value.