US 12,135,327 B2
Systems and methods for identifying HLA-associated tumor peptides
Robert Salzler, Tarrytown, NY (US)
Assigned to Regeneron Pharmaceuticals, Inc., Tarrytown, NY (US)
Filed by Regeneron Pharmaceuticals, Inc., Tarrytown, NY (US)
Filed on Jun. 28, 2023, as Appl. No. 18/343,260.
Application 18/343,260 is a division of application No. 16/361,342, filed on Mar. 22, 2019, granted, now 11,747,340, issued on Sep. 5, 2023.
Claims priority of provisional application 62/727,891, filed on Sep. 6, 2018.
Claims priority of provisional application 62/702,981, filed on Jul. 25, 2018.
Claims priority of provisional application 62/647,653, filed on Mar. 24, 2018.
Prior Publication US 2023/0417757 A1, Dec. 28, 2023
Int. Cl. G01N 33/68 (2006.01); C07K 1/22 (2006.01); C07K 1/36 (2006.01); C40B 30/04 (2006.01); G01N 33/569 (2006.01)
CPC G01N 33/6842 (2013.01) [C07K 1/22 (2013.01); C07K 1/36 (2013.01); C40B 30/04 (2013.01); G01N 33/56977 (2013.01); G01N 33/6818 (2013.01); G01N 33/6848 (2013.01); G01N 2333/70539 (2013.01)] 20 Claims
OG exemplary drawing
 
1. A method of determining amino acid sequences of peptides that bind to an MHC-I or HLA-I complex or an MHC-II or HLA-II complex, comprising:
obtaining a sample comprising unlabeled isolated peptides from an MHC-I or HLA-I complex or an MHC-II or HLA-II complex;
adding one or more labeled peptides to the sample to form a mixed sample comprising the labeled peptides and the unlabeled isolated peptides;
continuously analyzing the mixed sample with an LC-MS/MS system to produce survey mass spectrometry scans of the sample, wherein detection of the one or more labeled peptides triggers a target label filter causing the system to scan isolated unlabeled peptides with a second mass spectrometer until the LC-MS/MS system does not detect the one or more labeled peptides.