CPC G01N 33/58 (2013.01) [C07D 345/00 (2013.01); C07D 421/06 (2013.01); C07H 15/04 (2013.01); G01N 33/569 (2013.01); G01N 2458/15 (2013.01); G01N 2560/00 (2013.01)] | 21 Claims |
1. A kit capable of mass detection assay, the kit comprising:
a plurality of mass tags, each mass tag comprising a tellurophene compound of the below formula (I);
wherein
A is a naturally occurring isotope of Te;
R1 is selected from H, unsubstituted or substituted C1-C20alkyl, unsubstituted or substituted C3-C20cycloalkyl, unsubstituted or substituted aryl and an electron withdrawing group;
L is C1-30alkylene, unsubstituted or substituted with one or more substituents, and/or optionally interrupted with one or more heteromoieties independently selected from O, S, NR7, and/or optionally interrupted with one or more of C(O) and C(S);
R7 is independently selected from H, PG and C1-6alkyl;
X is a reactive functional group selected from halo, OH, Ots, Oms, C(O)H, C(O)OR8, C(O)NR9R10, O—C(O)—OR11, O—C(O)—NR12, C(O)ONR13R14, C(O)R15, C(O)SR16, NR17R18, or a maleimide,
R8 is selected from H, C1-6alkyl, aryl and C1-6alkylenearyl, wherein the latter three groups are unsubstituted or substituted with one or more of halo and NO2;
R9 and R10 are independently selected from H, C1-6alkyl, aryl and C1-6alkylenearyl, wherein the latter three groups are unsubstituted or substituted with one or more of halo and NO2, or
R9 and R10, together with the N atom to which they are bonded, form a 4 to 12 membered monocyclic or bicyclic, saturated or unsaturated ring unsubstituted or substituted with one or more ═O, ═S, halo and C1-6alkyl;
R11 is selected from C1-6alkyl, aryl and C1-6alkylenearyl, wherein the latter three groups are unsubstituted or substituted with one or more of halo and NO2;
R12 is selected from C1-6alkyl, aryl and C1-6alkylenearyl, wherein the latter three groups are unsubstituted or substituted with one or more of halo and NO2;
R13 and R14 are independently selected from H, C1-6alkyl, aryl and C1-6alkylenearyl, wherein the latter three groups are unsubstituted or substituted with one or more of halo and NO2, or
R13 and R14, together with the N atom to which they are bonded, form a 4 to 12 membered monocyclic or bicyclic, saturated or unsaturated ring unsubstituted or substituted with one or more ═O, ═S, halo and C1-6alkyl;
R15 is halo;
R16 is selected from C1-6alkyl, aryl and C1-6alkylenearyl, wherein the latter three groups are unsubstituted or substituted with one or more of halo and NO2;
R17 and R18 are independently selected from H, C(O)C1-6 alkyl, C1-6alkyl, aryl and C1-6alkylenearyl, wherein the latter three groups are unsubstituted or substituted with one or more of halo and NO2, or R17 and R18, together with the N atom to which they are bonded, form a 4 to 12 membered monocyclic or bicyclic, saturated or unsaturated ring unsubstituted or substituted with one or more ═O, ═S, halo and C1-6alkyl; and
one or more available hydrogens are optionally replaced with D;
or a salt and/or solvate thereof;
with proviso that when X is C(O)OR19 and R19 is H or C1-6alkyl, L is not C1-8alkylene; and
when X is OH, L is not C1-2alkylene; and
wherein each mass tag comprises a distinct tellurium isotope.
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