US 12,135,322 B2
In-vitro method for determining a cell type of a white blood cell without labeling
Lukas Richter, Hirschaid (DE); Oliver Hayden, Moosburg (DE); Matthias Ugele, Landshut (DE); Markus Weniger, Nuremberg (DE); Oliver Schmidt, Erlangen (DE); and Manfred Stanzel, Berching (DE)
Assigned to Siemens Healthineers AG, Erlangen (DE)
Appl. No. 16/980,835
Filed by Siemens Healthcare GmbH, Erlangen (DE)
PCT Filed Mar. 11, 2019, PCT No. PCT/EP2019/055965
§ 371(c)(1), (2) Date Sep. 14, 2020,
PCT Pub. No. WO2019/175082, PCT Pub. Date Sep. 19, 2019.
Claims priority of application No. 18162033 (EP), filed on Mar. 15, 2018.
Prior Publication US 2021/0365667 A1, Nov. 25, 2021
Int. Cl. G06T 7/00 (2017.01); G01N 33/49 (2006.01); G03H 1/00 (2006.01); G03H 1/04 (2006.01); G06F 18/2135 (2023.01); G06T 7/62 (2017.01); G06T 7/64 (2017.01); G06T 7/66 (2017.01); G06V 10/77 (2022.01); G06V 20/69 (2022.01); G06V 20/80 (2022.01)
CPC G01N 33/49 (2013.01) [G03H 1/0005 (2013.01); G03H 1/0443 (2013.01); G06F 18/2135 (2023.01); G06T 7/0012 (2013.01); G06T 7/62 (2017.01); G06T 7/64 (2017.01); G06T 7/66 (2017.01); G06V 10/7715 (2022.01); G06V 20/693 (2022.01); G06V 20/698 (2022.01); G03H 2001/005 (2013.01); G03H 2210/55 (2013.01); G06T 2207/10056 (2013.01); G06T 2207/30024 (2013.01); G06V 2201/03 (2022.01)] 14 Claims
 
1. An in-vitro method for determining a cell type of a white blood cell in a biological sample without labeling, the method comprising:
imaging the white blood cell using microscopy apparatus;
ascertaining physical parameters of the white blood cell from an image of the white blood cell by an automated image analysis; and
determining the cell type of the white blood cell based on the physical parameters and principal component analysis parameters, wherein the principal component analysis parameters comprise linear combinations of at least some of the physical parameters.