US 12,135,315 B2
Method for analyzing metalloprotein in biological sample
Satoshi Yamaki, Kyoto (JP); and Yun Zou, Beijing (CN)
Assigned to SHIMADZU CORPORATION, Kyoto (JP)
Appl. No. 17/047,216
Filed by Shimadzu Corporation, Kyoto (JP)
PCT Filed Apr. 12, 2019, PCT No. PCT/JP2019/015950
§ 371(c)(1), (2) Date Mar. 5, 2021,
PCT Pub. No. WO2019/198811, PCT Pub. Date Oct. 17, 2019.
Claims priority of application No. 201810332262.6 (CN), filed on Apr. 13, 2018.
Prior Publication US 2021/0215650 A1, Jul. 15, 2021
Int. Cl. G01N 30/74 (2006.01); G01N 30/02 (2006.01); G01N 30/06 (2006.01); G01N 30/72 (2006.01); G01N 30/88 (2006.01)
CPC G01N 30/74 (2013.01) [G01N 30/72 (2013.01); G01N 30/88 (2013.01); G01N 2030/027 (2013.01); G01N 2030/065 (2013.01)] 7 Claims
OG exemplary drawing
 
1. A method for analyzing a metalloprotein in a biological sample, the metalloprotein being a complex in which a biomolecule and a metal element bind to each other, the method comprising:
treating the biological sample that has been subjected to a pretreatment by liquid chromatography to separate the metalloprotein;
detecting the separated metalloprotein by a UV detector;
analyzing the separated metalloprotein by inductively coupled plasma mass spectrometry after detecting the separated metalloprotein by the UV detector;
comparing a detection signal of the UV detector with a detection signal of the inductively coupled plasma mass spectrometry; and
determining that the inductively coupled plasma mass spectrometry is failed when an intensity of the detection signal of the inductively coupled plasma mass spectrometry is smaller than an intensity of the detection signal of the UV detector,
wherein an ammonium acetate solution is used as a mobile phase.