US 12,135,300 B2
Single cell analysis using secondary ion mass spectrometry
Garry P. Nolan, Redwood City, CA (US); Sean C. Bendall, San Mateo, CA (US); and Robert M. Angelo, Menlo Park, CA (US)
Assigned to THE BOARD OF TRUSTEES OF THE LELAND STANFORD JUNIOR UNIVERSITY, Stanford, CA (US)
Filed by The Board of Trustees of the Leland Stanford Junior University, Stanford, CA (US)
Filed on Oct. 6, 2020, as Appl. No. 17/064,311.
Application 17/064,311 is a continuation of application No. 16/173,897, filed on Oct. 29, 2018, abandoned.
Application 16/173,897 is a continuation of application No. 15/679,044, filed on Aug. 16, 2017, granted, now 10,114,004, issued on Oct. 30, 2018.
Application 15/679,044 is a continuation of application No. 15/068,338, filed on Mar. 11, 2016, granted, now 9,766,224, issued on Sep. 19, 2017.
Claims priority of provisional application 62/138,322, filed on Mar. 25, 2015.
Prior Publication US 2021/0310970 A1, Oct. 7, 2021
This patent is subject to a terminal disclaimer.
Int. Cl. G01N 33/483 (2006.01); G01N 23/2258 (2018.01); G01N 33/50 (2006.01); G01N 33/68 (2006.01); H01J 49/00 (2006.01); H01J 49/14 (2006.01)
CPC G01N 23/2258 (2013.01) [G01N 33/4833 (2013.01); G01N 33/5005 (2013.01); G01N 33/5008 (2013.01); G01N 33/6848 (2013.01); H01J 49/0036 (2013.01); H01J 49/14 (2013.01); G01N 2458/15 (2013.01); H01J 49/142 (2013.01)] 19 Claims
 
12. A method of analyzing a test population of cells, comprising:
i) providing multiple individual cells of the test population on a substrate, wherein each cell is labeled with one or more mass tags;
ii) measuring, by scanning the individual cells on the substrate with an ionizing beam that generates ions from the cells, an abundance of the one or more mass tags for the individual cells and generating a set of data comprising, for each of the individual cells, mass tag abundance measurements;
iii) for each of the individual cells, comparing the set of data expressible as a histogram to reference information expressible as a reference histogram obtained from a reference population of cells to identify one or more cells of interest among the individual cells; and
iv) selectively removing the one or more cells of interest from the substrate.