US 12,135,292 B2
Detection method for detecting an analyte in a sample
Max Berg, Mannheim (DE); Daniel Sieffert, Mannheim (DE); Volker Tuerck, Berlin (DE); Simon Wetzel, Mannheim (DE); and Momme Winkelnkemper, Berlin (DE)
Assigned to Roche Diabetes Care, Inc., Indianapolis, IN (US)
Filed by Roche Diabetes Care, Inc., Indianapolis, IN (US)
Filed on Jan. 5, 2021, as Appl. No. 17/142,129.
Application 17/142,129 is a continuation of application No. PCT/EP2019/067815, filed on Jul. 3, 2019.
Claims priority of application No. 18182167 (EP), filed on Jul. 6, 2018.
Prior Publication US 2021/0131973 A1, May 6, 2021
Int. Cl. B01L 3/00 (2006.01); B01F 23/00 (2022.01); B01F 23/41 (2022.01); B01F 101/23 (2022.01); B23Q 17/24 (2006.01); C07K 14/705 (2006.01); C12M 1/34 (2006.01); C12Q 1/04 (2006.01); C12Q 1/18 (2006.01); C12Q 1/686 (2018.01); G01N 21/3577 (2014.01); G01N 21/359 (2014.01); G01N 21/39 (2006.01); G01N 21/45 (2006.01); G01N 21/64 (2006.01); G01N 21/77 (2006.01); G01N 21/78 (2006.01); G01N 27/414 (2006.01); G01N 30/12 (2006.01); G01N 30/68 (2006.01); G01N 30/70 (2006.01); G01N 30/72 (2006.01); G01N 30/88 (2006.01); G01N 33/00 (2006.01); G01N 33/18 (2006.01); G01N 33/50 (2006.01); G01N 33/53 (2006.01); G01N 33/543 (2006.01); G01N 33/68 (2006.01); G01N 33/74 (2006.01); G01N 35/00 (2006.01); G01N 35/10 (2006.01); G06T 7/00 (2017.01); G06T 7/90 (2017.01); H10K 10/46 (2023.01); H10K 85/00 (2023.01); H10K 85/20 (2023.01)
CPC G01N 21/78 (2013.01) [G06T 7/0014 (2013.01); G06T 7/90 (2017.01); G01N 2021/7759 (2013.01); G01N 2201/0221 (2013.01); G01N 2201/061 (2013.01); G06T 2207/10024 (2013.01); G06T 2207/10152 (2013.01); G06T 2207/30004 (2013.01)] 19 Claims
OG exemplary drawing
 
1. A method for detecting an analyte in a sample, comprising:
a) providing a mobile device having a camera, a processor and an illumination source;
b) providing a test strip comprising a test field having at least one test chemical for performing an optical detection reaction in the presence of the analyte;
c) applying the sample to the test field;
d) capturing a first image of a region of the test strip that includes at least a part of the test field to which the sample is applied, the first image being captured before step c) with the illumination source turned off;
e) capturing a second image of the region before step c) with the illumination source turned on;
f) capturing a third image of the region after step c) with the illumination source turned off;
g) capturing a fourth image of the region after step c) with the illumination source turned on;
wherein the processor of the mobile device is configured to perform steps of:
h) comparing corresponding images captured in steps d)-g) with the illumination source turned on and off, and determining differences in light intensities, step h) further comprising:
h1) deriving an item of color information of the part of the test field to which the sample is applied for each image to be compared;
h2) transforming the item of color information into an item of light intensity information by using at least one camera-specific transmission function;
h3) determining the differences in light intensities by using the items of light intensity information of corresponding images with the illumination source turned on and off;
i) determining the concentration of the analyte in the sample using the images captured in steps d)-g) and the result of step h).