US 12,135,288 B2
Methods for ultraviolet excitation microscopy of biological surfaces
Stavros G. Demos, Pittsford, NY (US); and Chi Huang, Foster City, CA (US)
Assigned to University of Rochester, Rochester, NY (US)
Filed by UNIVERSITY OF ROCHESTER, Rochester, NY (US)
Filed on Nov. 21, 2023, as Appl. No. 18/515,408.
Application 18/515,408 is a continuation of application No. 16/964,831, previously published as PCT/US2019/015103, filed on Jan. 25, 2019.
Claims priority of provisional application 62/622,358, filed on Jan. 26, 2018.
Claims priority of provisional application 62/642,730, filed on Mar. 14, 2018.
Prior Publication US 2024/0085330 A1, Mar. 14, 2024
Int. Cl. G01N 21/64 (2006.01); G01N 1/30 (2006.01)
CPC G01N 21/6458 (2013.01) [G01N 1/30 (2013.01); G01N 2001/302 (2013.01)] 16 Claims
OG exemplary drawing
 
1. A method of imaging a stained sample using an ultraviolet surface excitation microscopy system, the method comprising:
(a) using the ultraviolet surface excitation microscopy system to expose the stained sample to an ultraviolet light, wherein the stained sample is stained with a first fluorescing stain characterized by a first light emission spectrum when subjected to the ultraviolet light and a second fluorescing stain characterized by a second light emission spectrum when subjected to the ultraviolet light, wherein the first emission spectrum is different from the second emission spectrum; and
(b) using the ultraviolet surface excitation microscopy system to capture an image of the stained sample exposed to the ultraviolet light;
(c) digitally processing the captured image; and
(d) displaying the digitally processed image, wherein the displayed image includes features associated with the first fluorescing stain displayed at a first intensity and features associated with the second fluorescing stain displayed at a second intensity, wherein the first and second intensities are approximately the same.