US 12,135,272 B2
Multi-color flow cytometric analysis of samples with low cell numbers
Albert Mach, San Jose, CA (US); Smita Ghanekar, Fremont, CA (US); and Marja Suni, Los Gatos, CA (US)
Assigned to BECTON, DICKINSON AND COMPANY, Franklin Lakes, NJ (US)
Filed by Becton, Dickinson and Company, Franklin Lakes, NJ (US)
Filed on Apr. 1, 2020, as Appl. No. 16/837,900.
Application 16/837,900 is a division of application No. 15/371,088, filed on Dec. 6, 2016, granted, now 10,648,900.
Claims priority of provisional application 62/387,434, filed on Dec. 23, 2015.
Prior Publication US 2020/0225143 A1, Jul. 16, 2020
Int. Cl. G01N 15/1404 (2024.01); G01N 15/14 (2006.01); G01N 15/1433 (2024.01); G01N 15/01 (2024.01); G01N 15/10 (2006.01)
CPC G01N 15/1433 (2024.01) [G01N 15/1404 (2013.01); G01N 15/1425 (2013.01); G01N 15/01 (2024.01); G01N 2015/1006 (2013.01); G01N 2015/142 (2013.01); G01N 2015/1486 (2013.01)] 32 Claims
OG exemplary drawing
 
1. A flow cytometer for analyzing a biological sample assay composition, the flow cytometer comprising:
a sample input;
a sample concentrator integrated into a sample flow system of the flow cytometer and configured to produce a processed biological sample assay composition, the sample concentrator comprising:
an acoustic separator comprising:
a flow channel comprising an inlet and an outlet; and
an acoustic field generator positioned adjacent to the flow channel and configured to produce an acoustic field in the flow channel; and
a feedback monitor comprising a detector positioned at the outlet of the flow channel, wherein the feedback monitor is configured to provide a sample concentrator adjustment feedback based on a measurement of the biological sample assay composition or a portion thereof; and
a flow cytometer analysis system configured to analyze the processed biological sample assay composition.
 
31. A flow cytometer for analyzing a biological sample assay composition, the flow cytometer comprising:
a sample input; and
a sample concentrator integrated into a sample flow system of the flow cytometer and configured to produce a processed biological sample assay composition, the sample concentrator comprising:
an acoustic separator comprising:
a flow channel comprising an inlet, an outlet, and a conduit, wherein the conduit is fluidically coupled to the outlet and is configured to re-direct the biological sample assay composition or a portion thereof back to the inlet; and
an acoustic field generator positioned adjacent to the flow channel and configured to produce an acoustic field in the flow channel; and
a feedback monitor comprising a detector positioned at the outlet of the flow channel, wherein the feedback monitor is configured to provide a sample concentrator adjustment feedback based on a measurement of the biological sample assay composition or a portion thereof; and
a flow cytometer analysis system configured to analyze the processed biological sample assay composition.