US 12,135,267 B2
In vitro method for diagnosing, stratifying, prognosing and/or monitoring a lung disease and/or a respiratory disease
Jérémy Patarin, Revel (FR)
Assigned to RHEONOVA, Grenoble (FR)
Appl. No. 17/598,791
Filed by RHEONOVA, Grenoble (FR)
PCT Filed Mar. 30, 2020, PCT No. PCT/EP2020/058982
§ 371(c)(1), (2) Date Sep. 27, 2021,
PCT Pub. No. WO2020/193811, PCT Pub. Date Oct. 1, 2020.
Claims priority of application No. 1903302 (FR), filed on Mar. 28, 2019.
Prior Publication US 2022/0170836 A1, Jun. 2, 2022
Int. Cl. G01N 33/487 (2006.01); G01N 11/14 (2006.01)
CPC G01N 11/142 (2013.01) [G01N 33/487 (2013.01)] 21 Claims
OG exemplary drawing
 
1. An in vitro method for diagnosing, stratifying, prognosing and/or monitoring a lung disease and/or a respiratory disease, comprising:
(i) using a rheological measurement apparatus to establish a deformation curve for a sample of mucus A of a subject, the deformation curve comprising a linear zone, representing a linear deformation of the sample, and a non-linear zone, representing a non-linear deformation of the sample; wherein said sample of mucus A is placed in the rheological measurement apparatus between two slides rotationally moveable with respect to each other;
(ii) measuring a value Anl of at least one first rheological parameter on said a sample of mucus A, in the non-linear zone of said deformation curve; and
(iii) measuring a value Al of at least one second rheological parameter on said sample of mucus A, in the linear zone of said deformation curve;
the at least one first rheological parameter measured in the non-linear zone comprises at least critical stress (σc), and
the at least one second rheological parameter measured in the linear zone comprises at least one of complex modulus (G*), elasticity modulus (G′), viscosity modulus (G″), and damping factor (tan δ).