US 12,134,837 B2
Fusion imaging gene and lentiviral expression plasmid, lentivirus, cell, preparation methods and applications thereof
Zhiqiang Liu, Beijing (CN); Zengqiang Yuan, Beijing (CN); Cui Wang, Beijing (CN); Xiaowen Xing, Beijing (CN); Bingshui Xiu, Beijing (CN); and Shihong Liu, Beijing (CN)
Assigned to ACADEMY OF MILITARY MEDICAL SCIENCES, ACADEMY OF MILITARY SCIENCES OF CHINESE PLA, Beijing (CN); and BEIJING JIUYU ONCOLGY CO., LTD., Beijing (CN)
Filed by ACADEMY OF MILITARY MEDICAL SCIENCES, ACADEMY OF MILITARY SCIENCES OF CHINESE PLA, Beijing (CN); and BEIJING JIUYU ONCOLGY CO., LTD., Beijing (CN)
Filed on Feb. 1, 2024, as Appl. No. 18/430,589.
Application 18/430,589 is a continuation of application No. PCT/CN2022/080421, filed on Mar. 11, 2022.
Claims priority of application No. 202110882190.4 (CN), filed on Aug. 2, 2021.
Prior Publication US 2024/0167021 A1, May 23, 2024
Int. Cl. C40B 40/08 (2006.01); C12N 15/10 (2006.01); C12N 15/65 (2006.01); C12N 15/86 (2006.01)
CPC C40B 40/08 (2013.01) [C12N 15/1086 (2013.01); C12N 15/65 (2013.01); C12N 15/86 (2013.01); C12N 2740/15022 (2013.01); C12N 2740/15043 (2013.01)] 17 Claims
OG exemplary drawing
 
1. A fusion imaging gene, wherein the fusion imaging gene comprises a bioluminescence imaging gene, a fluorescent protein gene and a calcium imaging gene, and the three genes are linked by linkers;
the bioluminescence imaging gene is selected from a Nanoluc gene;
the fluorescent protein gene is selected from a mRuby2 gene;
the calcium imaging gene is selected from a Gcamp6f gene;
the Nanoluc gene and the mRuby2 gene are linked through Linker 1, as shown in SEQ ID NO.8; the mRuby2 gene and the Gcamp6f gene are linked through Linker 2, as shown in SEQ ID NO.9;
a nucleotide sequence of the fusion imaging gene is shown in SEQ ID NO.1.