| CPC C12Q 1/6832 (2013.01) | 21 Claims |
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1. A method for enriching a population of nucleic acid target sequences in a sample comprising:
(a) providing a sample of nucleic acid molecules comprising a plurality of target nucleic acid sequences and a plurality of off-target nucleic acid sequences;
(b) hybridizing the sample to a panel of nucleic acid probes that are complementary to the plurality of target nucleic acid sequences under hybridization conditions to generate a probe/target complex;
(c) selectively immobilizing the probe/target complex to form an immobilized probe/target complex and adding a melt buffer;
(d) heating the immobilized probe/target complex and the melt buffer to a temperature at or below the aggregate Tm of the probe/target complex for a period of time sufficient to disassociate the off-target nucleic acid sequences; and
(e) washing the immobilized probe/target complex to remove non-hybridized nucleic acid sequences and off-target nucleic acid sequences from the hybridized plurality of target nucleic acid sequences, thereby enriching for the plurality of target nucleic acid sequences in the sample;
wherein step (d) is performed prior to step (e).
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