	US 12,134,793 B2
	Recombinant microorganism having increased ability to produce hydrophobic material and cell-membrane engineering method for preparation thereof
Sang Yup Lee, Daejeon (KR); Dongsoo Yang, Daejeon (KR); and Seon Young Park, Daejeon (KR)
Assigned to KOREA ADVANCED INSTITUTE OF SCIENCE AND TECHNOLOGY, Daejeon (KR)
Filed by KOREA ADVANCED INSTITUTE OF SCIENCE AND TECHNOLOGY, Daejeon (KR)
Filed on Nov. 1, 2021, as Appl. No. 17/515,614.
Claims priority of application No. 10-2020-0144521 (KR), filed on Nov. 2, 2020.
Prior Publication US 2022/0136028 A1, May 5, 2022
Int. Cl. C12P 23/00 (2006.01); C07K 14/245 (2006.01); C12N 1/20 (2006.01); C12N 9/04 (2006.01); C12N 9/10 (2006.01); C12N 9/90 (2006.01); C12P 17/16 (2006.01)

CPC C12P 23/00 (2013.01) [C07K 14/245 (2013.01); C12N 1/20 (2013.01); C12N 9/0006 (2013.01); C12N 9/1029 (2013.01); C12N 9/1051 (2013.01); C12N 9/90 (2013.01); C12P 17/165 (2013.01); C12Y 101/01336 (2015.07); C12Y 204/01044 (2013.01); C12Y 204/01129 (2013.01); C12Y 501/0302 (2013.01)]

8 Claims

1. A recombinant bacterium, obtained by engineering a hydrophobic-pigment-producing bacterium by: i) rfaD gene encoding ADP-L-glycero-D-manno-heptose-6-epimerase and rffD gene encoding UDP-N-acetyl-D-mannosamine dehydrogenase are suppressed, ii) rfaI gene encoding lipopolysaccharide 1,3-galactosyltransferase is suppressed, and cav1 gene encoding caveolin-1 is introduced or overexpressed, or iii) rfaD gene and rffD gene are suppressed and cav1 gene is introduced or overexpressed, and the engineered bacterium is increasingly capable of producing a hydrophobic pigment.