	US 12,134,792 B1
	Process for production of a soluble and insoluble collagen product from mammalian dermis tissue
Mohammad Z. Albanna, Chandler, AZ (US); and Nilabh S. Kajave, Tempe, AZ (US)
Assigned to Humabiologics, Inc., Phoenix, AZ (US)
Filed by Humabiologics, Inc., Phoenix, AZ (US)
Filed on May 3, 2024, as Appl. No. 18/654,723.
Claims priority of provisional application 63/463,579, filed on May 3, 2023.
Int. Cl. C12P 21/06 (2006.01); A23J 1/10 (2006.01); A61K 38/17 (2006.01); C07K 1/14 (2006.01); C07K 14/78 (2006.01); C12P 21/02 (2006.01)

CPC C12P 21/02 (2013.01)

14 Claims

1. A process for the production of a soluble collagen product and an insoluble collagen product from mammalian dermis tissue comprising:

a) providing minced dermis tissue from said mammalian dermis tissue:

b) removing lipids from the minced dermis tissue comprising:

i) providing a first lipid removal solution comprising:

chloroform;

methanol; and

hexane;

ii) washing the minced dermis tissue with said first lipid removal solution for a washing time of at least 8 hours;

c) providing an enzymatic solution comprising:

i) water; and

ii) amylase;

d) after washing in said first lipid removal solution, washing the minced dermis tissue in the enzymatic solution to produce an enzymatic solution washed tissue;

e) homogenizing the enzymatic solution washed tissue at temperature of no more than 40° C. to produce homogenized tissue;

f) providing extraction solution;

g) extracting both said soluble collagen and said insoluble collagen from the homogenized tissue by washing the homogenized tissue in said extraction solution to produce an extracted collagen; and

h) separating said soluble collagen and said insoluble collagen from said extracted collagen.