US 12,134,783 B2
Tissue processing apparatus and method for infusing bioactive agents into tissue
Subhabrata Bhattacharyya, Metuchen, NJ (US); David R. Kaes, Toms River, NJ (US); and Daniel A. Shimko, Germantown, TN (US)
Assigned to Warsaw Orthopedic, Inc., Warsaw, IN (US)
Filed by Warsaw Orthopedic, Inc., Warsaw, IN (US)
Filed on Feb. 3, 2021, as Appl. No. 17/166,491.
Application 17/166,491 is a division of application No. 15/232,275, filed on Aug. 9, 2016, granted, now 10,913,930.
Prior Publication US 2021/0180019 A1, Jun. 17, 2021
This patent is subject to a terminal disclaimer.
Int. Cl. C12N 5/077 (2010.01); A61F 2/46 (2006.01); A61L 27/54 (2006.01); G01N 1/31 (2006.01)
CPC C12N 5/0654 (2013.01) [A61F 2/4644 (2013.01); A61F 2250/0067 (2013.01); A61L 27/54 (2013.01); A61L 2430/02 (2013.01); A61L 2430/40 (2013.01); G01N 1/31 (2013.01); G01N 2001/315 (2013.01)] 10 Claims
OG exemplary drawing
 
1. A method for treating a bone tissue sample, comprising:
providing a controller; an enclosure defining a reaction chamber for accepting the tissue sample; a reagent supply system configured to receive at least one reagent and to dispense the at least one reagent into the reaction chamber in response to the controller, the at least one reagent comprising at least one bioactive agent; a gas evacuation assembly communicated to the reaction chamber and configured to pump gas from the reaction chamber in response to the controller;
a draining assembly communicated to the reaction chamber and configured to drain fluid from the reaction chamber in response to the controller; and a signal transmission system functionally interconnecting the controller with the reagent supply system, the gas evacuation assembly, and the draining assembly;
loading the controller with a treatment procedure programming for effecting a treatment procedure;
loading the reagent supply system with a first reagent of the at least one reagent;
disposing the bone tissue sample in the reaction chamber;
effecting application of the first reagent to the bone tissue sample by the controller activating the reagent supply system to dispense a predetermined amount of the first reagent into the reaction chamber in accordance with the treatment procedure programming;
effecting draining of the first reagent from the reaction chamber by the controller activating the draining assembly to drain the first reagent from the reaction chamber, after a predetermined time period has passed since the application of the first reagent to the bone biologic tissue sample, following the treatment procedure programming;
effecting evacuation of the reaction chamber to a predetermined pressure level by the controller activating the gas evacuation assembly to evacuate gas from the reaction chamber during the application of the first reagent to the bone tissue sample in accordance with the treatment procedure programming;
providing a carbon dioxide sensor communicated to the reaction chamber to detect a carbon dioxide level in the reaction chamber, which communicates the carbon dioxide level to the controller via the signal transmission system; and
maintaining a CO2 level in the reaction chamber below a predetermined CO2 level during the application of the first reagent to the bone tissue sample by the controller monitoring the CO2 level in the reaction chamber and activating the gas evacuation assembly in response to the CO2 level rising above the predetermined CO2 level to bring the CO2 level to or below the predetermined CO2 level in accordance with the treatment procedure programming, wherein the first reagent is an acid suitable for demineralizing the bone material or the first reagent is the at least one bioactive agent.