	US 12,134,775 B2
	Resistance to soybean cyst nematode through gene editing
Andrew Fister, Morrisville, NC (US); and Marisa Miller, Durham, NC (US)
Assigned to Pairwise Plants Services, Inc., Durham, NC (US)
Filed by Pairwise Plants Services, Inc., Durham, NC (US)
Filed on Feb. 19, 2021, as Appl. No. 17/180,369.
Claims priority of provisional application 62/979,757, filed on Feb. 21, 2020.
Prior Publication US 2021/0261978 A1, Aug. 26, 2021
Int. Cl. C12N 15/82 (2006.01); C07K 14/415 (2006.01); C12N 15/11 (2006.01)

CPC C12N 15/8285 (2013.01) [C07K 14/415 (2013.01); C12N 15/11 (2013.01); C12N 2310/20 (2017.05)]

14 Claims

1. A soybean plant or plant part thereof comprising at least one non-natural mutation in the C-terminal region of an endogenous Soluble NSF Attachment Protein18 (SNAP18) protein, wherein the endogenous SNAP18 protein comprises a sequence having at least 99% sequence identity to the amino acid sequence of SEQ ID NO:57, and the at least one mutation comprises a mutation of one or more amino acid residue(s) located at positions 281-290 with reference to amino acid position numbering of SEQ ID NO:57.