	US 12,134,771 B2
	Fusion protein and use thereof in base editing
Yajing Liu, Shanghai (CN); Shisheng Huang, Shanghai (CN); and Xingxu Huang, Shanghai (CN)
Assigned to ShanghaiTech University, Shanghai (CN)
Filed by ShanghaiTech University, Shanghai (CN)
Filed on Jan. 5, 2023, as Appl. No. 18/150,778.
Application 18/150,778 is a division of application No. 17/323,603, filed on May 18, 2021.
Prior Publication US 2023/0313205 A1, Oct. 5, 2023
Int. Cl. C12N 15/62 (2006.01); C07K 14/775 (2006.01); C12N 9/22 (2006.01)

CPC C12N 15/625 (2013.01) [C07K 14/775 (2013.01); C12N 9/22 (2013.01); C07K 2319/09 (2013.01)]

16 Claims

1. A fusion protein comprising a first fragment of a nCas9 protein, a chimeric insertion polypetide and a second fragment of the nCas9 protein from N-terminus to C-terminus, wherein the chimeric insertion polypetide is TadA-TadA*, wherein the TadA-TadA* is a polypeptide encoded by the nucleotide sequence shown in SEQ ID NO: 11; wherein

the first fragment of the nCas9 protein has an amino acid sequence as set forth in SEQ ID NO: 1; and

the second fragment of the nCas9 protein has an amino acid sequence as set forth in SEQ ID NO: 2.