	US 12,134,768 B2
	Structural domains of antisense RNA molecules up-regulating translation
Stefano Gustincich, Trieste (IT); Silvia Zucchelli, Trieste (IT); Peter Podbevsek, Trieste (IT); Janez Plavec, Trieste (IT); Piero Carninci, Yokohama (JP); Hazuki Takahashi, Trieste (IT); Toshio Yamazaki, Trieste (IT); Takako Ohyama, Trieste (IT); and Harshita Sharma, Trieste (IT)
Assigned to FONDAZIONE ISTITUTO ITALIANO DI TECNOLOGIA, (IT)
Appl. No. 16/967,254
Filed by Fondazione Istituto Italiano Di Tecnologia, Genoa (IT)
PCT Filed Feb. 5, 2019, PCT No. PCT/IB2019/050914 § 371(c)(1), (2) Date Aug. 4, 2020, PCT Pub. No. WO2019/150346, PCT Pub. Date Aug. 8, 2019.
Claims priority of application No. 102018000002411 (IT), filed on Feb. 5, 2018.
Prior Publication US 2021/0139898 A1, May 13, 2021
Int. Cl. C12N 15/113 (2010.01); C12N 15/67 (2006.01); C12Q 1/68 (2018.01)

CPC C12N 15/113 (2013.01) [C12N 15/67 (2013.01); C12N 2310/11 (2013.01); C12N 2310/531 (2013.01); C12N 2310/532 (2013.01); C12N 2320/11 (2013.01)]

8 Claims

1. A functional nucleic acid molecule comprising:

a target binding sequence comprising a sequence reverse complementary to a target mRNA sequence for which protein translation is to be enhanced; and

a regulatory sequence having a two-dimensional structure comprising a first stem-loop domain (SL-1) comprising a stem with 8 to 12 paired nucleotides and a loop comprising 5 to 9 nucleotides, wherein the regulatory sequence consists of a sequence having at least 90% identity with a sequence selected from the group consisting of SEQ ID NO:7, SEQ ID NO: 12 and SEQ ID NO: 1.