US 12,134,765 B2
Methods for cell-free DNA extraction for non-invasive prenatal screening
Ben Anderson, San Juan Capistrano, CA (US); Charles Strom, San Juan Capistrano, CA (US); David Tsao, San Juan Capistrano, CA (US); Yan Liu, San Juan Capistrano, CA (US); and Weimin Sun, San Juan Capistrano, CA (US)
Assigned to Quest Diagnostics Investments LLC, Secaucus, NJ (US)
Filed by Quest Diagnostics Investments LLC, Secaucus, NJ (US)
Filed on Oct. 31, 2022, as Appl. No. 17/977,716.
Application 17/977,716 is a continuation of application No. 15/770,855, granted, now 11,485,967, previously published as PCT/US2016/058640, filed on Oct. 25, 2016.
Claims priority of provisional application 62/246,421, filed on Oct. 26, 2015.
Prior Publication US 2023/0265414 A1, Aug. 24, 2023
This patent is subject to a terminal disclaimer.
Int. Cl. C12Q 1/6806 (2018.01); C12N 15/10 (2006.01); C12Q 1/686 (2018.01)
CPC C12N 15/1013 (2013.01) [C12Q 1/6806 (2013.01); C12Q 1/686 (2013.01); C12Q 2600/112 (2013.01); C12Q 2600/16 (2013.01); C12Q 2600/166 (2013.01)] 20 Claims
 
1. A method for improving the yield of cell-free DNA (cfDNA) isolated from a liquid biological sample from a subject comprising:
(a) contacting a liquid biological sample containing cfDNA with a plurality of magnetic beads that reversibly bind to DNA;
(b) separating the cfDNA-bound magnetic beads from the unbound components of the liquid biological sample using a magnet;
(c) transferring the cfDNA-bound magnetic beads to a vessel containing an elution solution; and
(d) separating the magnetic beads from the elution solution by contacting the vessel containing the magnetic beads in the elution solution to a magnetic plate that comprises a ring magnet, and collecting the elution solution containing the cfDNA, wherein the magnetic plate causes the magnetic beads to adhere to the vessel walls.