US 12,134,759 B2
Glomerulus on a chip to recapitulate glomerular filtration barrier
Laura Perin, Burbank, CA (US); Stefano Da Sacco, Glendale, CA (US); and Roger De Filippo, Glendale, CA (US)
Assigned to Children's Hospital Los Angeles, Los Angeles, CA (US)
Filed by Children's Hospital Los Angeles, Los Angeles, CA (US)
Filed on Oct. 19, 2023, as Appl. No. 18/490,493.
Application 18/490,493 is a continuation of application No. 16/870,480, filed on May 8, 2020, granted, now 11,840,683.
Claims priority of provisional application 62/846,112, filed on May 10, 2019.
Prior Publication US 2024/0158728 A1, May 16, 2024
This patent is subject to a terminal disclaimer.
Int. Cl. C12M 3/06 (2006.01); C12M 1/00 (2006.01); C12M 1/12 (2006.01); C12N 5/071 (2010.01); G01N 33/50 (2006.01)
CPC C12M 23/16 (2013.01) [C12M 25/02 (2013.01); C12M 29/10 (2013.01); C12N 5/0686 (2013.01); G01N 33/5088 (2013.01)] 20 Claims
OG exemplary drawing
 
1. A glomerulus on a chip (GOAC) device, comprising:
a first channel that includes a glomerular renal filtration barrier that includes:
a first monolayer comprised of podocytes; and
a second monolayer disposed on the first monolayer,
wherein the second monolayer is comprised of glomerular endothelial cells and the second monolayer contacts the first monolayer; and
a second channel in fluid communication with the first channel.