US 12,134,755 B2
Cell stimulation and culture platform using ultrasonic hologram
Jae Youn Hwang, Daegu (KR); and Moon Hwan Lee, Gyeongsan-si (KR)
Assigned to DAEGU GYEONGBUK INSTITUTE OF SCIENCE AND TECHNOLOGY, Daegu (KR)
Filed by DAEGU GYEONGBUK INSTITUTE OF SCIENCE AND TECHNOLOGY, Daegu (KR)
Filed on Oct. 6, 2022, as Appl. No. 17/960,999.
Claims priority of application No. 10-2021-0132152 (KR), filed on Oct. 6, 2021.
Prior Publication US 2023/0108076 A1, Apr. 6, 2023
Int. Cl. C12M 1/42 (2006.01); C12M 1/00 (2006.01); C12M 3/00 (2006.01); C12N 13/00 (2006.01); G01N 29/06 (2006.01); G03H 3/00 (2006.01)
CPC C12M 1/42 (2013.01) [C12M 23/58 (2013.01); C12M 35/04 (2013.01); C12N 13/00 (2013.01); G01N 29/0663 (2013.01); G03H 3/00 (2013.01)] 11 Claims
OG exemplary drawing
 
1. A cell stimulation and culture platform using an ultrasonic hologram, comprising:
a culture vessel in which a culture well with an open lower portion is formed;
a transmission sheet which is installed to cover a lower surface of the culture vessel and where a biological sample is seated on a surface portion corresponding to the culture well;
a platform body of which an inner space is filled with a liquid ultrasonic medium and an open upper end is covered by the transmission sheet;
an ultrasonic transducer that is installed at an inner lower end portion of the platform body in a state of being spaced apart from the biological sample by a set distance to generate ultrasonic waves upward; and
an ultrasonic hologram lens that is installed on the ultrasonic transducer, transmits the incident ultrasonic waves to a target surface on which the biological sample is located through the ultrasonic medium, and spatially modulates the phase of the ultrasonic waves using a surface structure designed to have different height distributions to focus the ultrasonic waves in a set pattern shape on the target surface.