US 12,134,754 B2
Well inserts with brittle membranes and method of manufacturing the same
Silvia Angeloni Suter, Saint-Blaise (CH); Kaspar Suter, Saint-Blaise (CH); Eric Marguet, Saint-Imier (CH); Sylvain Bergerat, Le Russey (FR); and Charlotte Voutat, Bole (CH)
Assigned to SiMPLInext SA, Bienne (CH)
Filed by SIMPLInext SA, Bienne (CH)
Filed on Jan. 5, 2023, as Appl. No. 18/150,571.
Application 18/150,571 is a division of application No. 15/573,708, granted, now 11,572,536, previously published as PCT/EP2016/060468, filed on May 10, 2016.
Claims priority of application No. PCT/EP2015/060312 (WO), filed on May 11, 2015.
Prior Publication US 2023/0151312 A1, May 18, 2023
Int. Cl. C12M 1/12 (2006.01); B29C 45/00 (2006.01); C08L 23/00 (2006.01); C12M 1/00 (2006.01); C12M 3/06 (2006.01)
CPC C12M 1/125 (2013.01) [B29C 45/0001 (2013.01); B29C 45/0046 (2013.01); C12M 3/062 (2013.01); C12M 23/00 (2013.01); C12M 25/04 (2013.01); C08L 23/00 (2013.01); C08L 2203/02 (2013.01)] 9 Claims
OG exemplary drawing
 
1. A well insert (10) for cell culture, comprising:
a membrane support (10a), made of polyolefin material and comprising a hanger (70) having a first open end comprising a flange (11) sized to interface with a well of a microplate so as to suspend the well insert (10) therein, a second end (12), a first intermediate section comprising an impermeable sidewall (13) adjacent to the second end, a second intermediate section joining the impermeable wall to the flange, said second intermediate section comprising a plurality of openings (14) divided by connecting elements (15); and
a permeable membrane (1), transparent in both air and water, for supporting a tissue culture, the permeable membrane (1) being arranged at said lower end of the hanger (70) and sealed thereto, the permeable membrane (1) having a thickness of less than 10 μm, or in the range of 0.1 to 10 μm, being of a material which does not exhibit a plastic deformation regime,
wherein the membrane support (10a) comprises a seal (65) arranged at said second end of said hanger (70), and wherein said membrane support (10a) further comprises an end piece (60) releasably clipped to said hanger (70) so as to support said permeable membrane (1) between said end piece (60) and said hanger (70) in contact with said seal, said end piece (60) being arranged so as to cause said permeable membrane (1) to compress said seal upon clipping of said end piece on said hanger, said end piece (60) comprising a plurality of arms (63) extending towards said openings (14) of the hanger, and comprising first clipping element (64) each interfacing with the lower edge of corresponding openings (14) provided on said hanger (70).