US 12,134,751 B2
Bioactive protein-polymer compositions for stain removal
Andreas Buthe, Steinfurt (DE); Ping Wang, North Oaks, MN (US); Songtao Wu, Ann Arbor, MI (US); Hongfei Jia, Ann Arbor, MI (US); Masahiko Ishii, Okazaki (JP); and Minjuan Zhang, Ann Arbor, MI (US)
Assigned to REGENTS OF THE UNIVERSITY OF MINNESOTA, Minneapolis, MN (US); and TOYOTA MOTOR CORPORATION, Toyota (JP)
Filed by Toyota Motor Corporation, Toyota (JP); and Regents of the University of Minnesota, Minneapolis, MN (US)
Filed on Jun. 1, 2023, as Appl. No. 18/327,433.
Application 18/327,433 is a division of application No. 16/900,386, filed on Jun. 12, 2020, granted, now 11,692,156.
Application 16/900,386 is a continuation of application No. 15/810,700, filed on Nov. 13, 2017, granted, now 11,015,149, issued on May 25, 2021.
Application 15/810,700 is a continuation in part of application No. 15/193,242, filed on Jun. 27, 2016, granted, now 10,767,141, issued on Sep. 8, 2020.
Application 15/193,242 is a continuation of application No. 13/567,341, filed on Aug. 6, 2012, granted, now 9,388,370, issued on Jul. 12, 2016.
Application 13/567,341 is a continuation in part of application No. 12/820,101, filed on Jun. 21, 2010, granted, now 8,796,009, issued on Aug. 5, 2014.
Application 15/810,700 is a continuation in part of application No. 14/812,087, filed on Jul. 29, 2015, granted, now 10,563,094, issued on Feb. 18, 2020.
Application 14/812,087 is a continuation of application No. 13/229,277, filed on Sep. 9, 2011, granted, now 9,121,016, issued on Sep. 1, 2015.
Prior Publication US 2023/0374420 A1, Nov. 23, 2023
This patent is subject to a terminal disclaimer.
Int. Cl. C11D 3/386 (2006.01); C08G 18/62 (2006.01); C08G 18/79 (2006.01); C09D 175/04 (2006.01); C11D 3/04 (2006.01); C11D 3/20 (2006.01); C11D 3/37 (2006.01); C11D 3/43 (2006.01); C11D 7/04 (2006.01); C11D 7/26 (2006.01); D06L 4/40 (2017.01); D06M 16/00 (2006.01)
CPC C11D 3/38618 (2013.01) [C08G 18/6225 (2013.01); C08G 18/792 (2013.01); C09D 175/04 (2013.01); C11D 3/04 (2013.01); C11D 3/044 (2013.01); C11D 3/2003 (2013.01); C11D 3/201 (2013.01); C11D 3/37 (2013.01); C11D 3/386 (2013.01); C11D 3/38627 (2013.01); C11D 3/43 (2013.01); C11D 7/04 (2013.01); C11D 7/261 (2013.01); C12Y 304/24027 (2013.01); D06L 4/40 (2017.01); D06M 16/003 (2013.01); C11D 2111/18 (2024.01)] 12 Claims
 
1. A method of facilitating removal of an organic stain from a substrate or coating comprising:
providing a substrate or coating formed of an organic crosslinkable polymer resin;
associating a lipase with said substrate or said coating, said lipase capable of enzymatically degrading a component of an organic stain, wherein said lipase is uniformly dispersed in said substrate or said coating in protein aggregates having an average diameter of 10 micrometers or less; and
facilitating removal of an organic stain from said substrate or said coating by rinsing.