US 12,134,656 B2
Dickkopf-1 variant antibodies and methods of use
Aaron Sato, Burlingame, CA (US); Tom Yuan, San Francisco, CA (US); Linya Wang, Milpitas, CA (US); and Fumiko Axelrod, Palo Alto, CA (US)
Assigned to Twist Bioscience Corporation, South San Francisco, CA (US)
Filed by Twist Bioscience Corporation, South San Francisco, CA (US)
Filed on Nov. 17, 2022, as Appl. No. 18/056,679.
Claims priority of provisional application 63/379,634, filed on Oct. 14, 2022.
Claims priority of provisional application 63/374,497, filed on Sep. 2, 2022.
Claims priority of provisional application 63/286,522, filed on Dec. 6, 2021.
Claims priority of provisional application 63/280,840, filed on Nov. 18, 2021.
Prior Publication US 2023/0312749 A1, Oct. 5, 2023
Int. Cl. C07K 16/32 (2006.01)
CPC C07K 16/32 (2013.01) [C07K 2317/565 (2013.01); C07K 2317/569 (2013.01); C07K 2317/92 (2013.01)] 20 Claims
 
1. An antibody or antibody fragment that binds to dickkopf-1 (DKK1) comprising a VHH domain, wherein the VHH domain comprises:
a) a heavy chain CDR1 comprising the amino acid sequence of SEQ ID NO: 66, a heavy chain CDR2 comprising the amino acid sequence of SEQ ID NO: 164, and a heavy chain CDR3 comprising the amino acid sequence of SEQ ID NO: 262;
b) a heavy chain CDR1 comprising the amino acid sequence of SEQ ID NO: 69, a heavy chain CDR2 comprising the amino acid sequence of SEQ ID NO: 167, and a heavy chain CDR3 comprising the amino acid sequence of SEQ ID NO: 265;
c) a heavy chain CDR1 comprising the amino acid sequence of SEQ ID NO: 94, a heavy chain CDR2 comprising the amino acid sequence of SEQ ID NO: 192, and a heavy chain CDR3 comprising the amino acid sequence of SEQ ID NO: 290;
d) a heavy chain CDR1 comprising the amino acid sequence of SEQ ID NO: 98, a heavy chain CDR2 comprising the amino acid sequence of SEQ ID NO: 196, and a heavy chain CDR3 comprising the amino acid sequence of SEQ ID NO: 294;
e) a heavy chain CDR1 comprising the amino acid sequence of SEQ ID NO: 977, a heavy chain CDR2 comprising the amino acid sequence of SEQ ID NO: 1391, and a heavy chain CDR3 comprising the amino acid sequence of SEQ ID NO: 1805; or
f) a heavy chain CDR1 comprising the amino acid sequence of SEQ ID NO: 1305, a heavy chain CDR2 comprising the amino acid sequence of SEQ ID NO: 1719, and a heavy chain CDR3 comprising the amino acid sequence of SEQ ID NO: 2133.