US 12,134,095 B2
Apparatus and methods for performing microfluidic-based biochemical assays
Armando Paredes, Lincoln, MA (US)
Filed by SiPhox, Inc., Burlington, MA (US)
Filed on Mar. 15, 2023, as Appl. No. 18/121,712.
Claims priority of provisional application 63/302,365, filed on Jan. 24, 2022.
Prior Publication US 2023/0264195 A1, Aug. 24, 2023
Int. Cl. B01L 3/00 (2006.01)
CPC B01L 3/502715 (2013.01) [B01L 2200/025 (2013.01); B01L 2200/16 (2013.01); B01L 2300/0654 (2013.01); B01L 2300/0883 (2013.01); B01L 2400/0406 (2013.01); B01L 2400/08 (2013.01)] 18 Claims
OG exemplary drawing
 
9. A method for performing microfluidic-based biochemical assays, the method comprising:
positioning a sensor device by using an alignment feature of a microfluidic device, the microfluidic device comprising a microfluidic feature comprising a reservoir configured to contain a fluid; and the alignment feature is is not in physical contact with the microfluidic feature;
flowing, in a microfluidic environment, the fluid through the sensor device, wherein the microfluidic environment comprises:
a bubble trap fluidically connected to the at least a microfluidic feature; and a flow component fluidically connected to the microfluidic feature, wherein the sensor device comprises a sensor interface comprising a porous member configured to control one or more flows, and the flow component comprises: a passive flow component configured to initiate a passive flow process; and an active flow component configured to initiate an active flow process, wherein the active flow component comprises a bubble barrier, wherein the bubble barrier is configured to prevent pollutants between the microfluidic feature and the active flow component and to maintain a consistent flow of the active flow process by buffering a steeped action movement of the plunger wherein the active flow component comprises a barrel and a plunger disposed within the barrel, wherein the plunger performs a movement inside the barrel wherein the movement of the plunger inside the barrel initiates the active flow process; and
detecting, using an optical device of the a sensor device configured to communicate with the fluid, wherein the optical device comprises a resonator, an optical property using the resonator.