US 12,133,933 B2
Growth factor transduced cell-loaded ceramic scaffold for bone regeneration and repair
Jay R. Lieberman, Los Angeles, CA (US); William Pannell, Los Angeles, CA (US); Yong Chen, Los Angeles, CA (US); Xuan Song, Los Angeles, CA (US); and Sofia Bougioukli, Los Angeles, CA (US)
Assigned to UNIVERSITY OF SOUTHERN CALIFORNIA, Los Angeles, CA (US)
Filed by UNIVERSITY OF SOUTHERN CALIFORNIA, Los Angeles, CA (US)
Filed on Jun. 11, 2020, as Appl. No. 16/899,363.
Application 16/899,363 is a continuation in part of application No. 16/337,893, previously published as PCT/US2017/054609, filed on Sep. 29, 2017.
Claims priority of provisional application 62/401,745, filed on Sep. 29, 2016.
Prior Publication US 2020/0316258 A1, Oct. 8, 2020
Int. Cl. A61L 27/38 (2006.01); A61L 27/10 (2006.01); A61L 27/12 (2006.01); A61L 27/22 (2006.01); A61L 27/54 (2006.01); B28B 1/00 (2006.01); B29C 64/379 (2017.01); B29K 509/02 (2006.01); B29L 31/00 (2006.01); B33Y 10/00 (2015.01); B33Y 40/20 (2020.01); B33Y 70/00 (2020.01); B33Y 80/00 (2015.01); B29C 64/129 (2017.01)
CPC A61L 27/3847 (2013.01) [A61L 27/10 (2013.01); A61L 27/12 (2013.01); A61L 27/227 (2013.01); A61L 27/3821 (2013.01); A61L 27/3834 (2013.01); A61L 27/54 (2013.01); B28B 1/001 (2013.01); B29C 64/379 (2017.08); B33Y 40/20 (2020.01); B33Y 70/00 (2014.12); B33Y 80/00 (2014.12); A61L 2300/414 (2013.01); A61L 2430/02 (2013.01); B29C 64/129 (2017.08); B29K 2509/02 (2013.01); B29L 2031/7532 (2013.01); B33Y 10/00 (2014.12)] 8 Claims
OG exemplary drawing
 
1. A method for repairing a bone defect of a patient, comprising:
providing a 3D model of a scaffold for bridging the bone defect;
providing a ceramic scaffold comprising calcium and phosphate based on the 3D model, wherein the ceramic scaffold comprises a tube-like structure that comprises an inner surface that matches the outer surface of a healthy bone,
wherein the ceramic scaffold is custom shaped to match morphology of healthy bone that surrounds the bone defect;
transducing any one or more of mesenchymal stem cells, bone marrow cells, fibroblasts, adipose-derived cells, umbilical cord cells, and muscle cells with a lentiviral vector system comprising (i) a trans-activator vector with a multiplicity of infection (MOI) of 3 and (ii) a vector encoding a growth factor essential for bone formation with a MOI of 3;
loading any one or more of mesenchymal stem cells, bone marrow cells, fibroblasts, adipose-derived cells, umbilical cord cells, and muscle cells, transduced with the lentiviral vector system, on the inner surface of the ceramic scaffold;
placing the ceramic scaffold into the bone defect of the patient; and
stabilizing the ceramic scaffold in the patient using a biocompatible metal plate or rod, until the bone defect is repaired.