US 12,133,888 B2
Engineered influenza polynucleotides, viruses, vaccines and methods of making and using the same
Nicholas Scott Heaton, Durham, NC (US); Alfred Theodore Harding, Durham, NC (US); and Brook Elizabeth Heaton, Durham, NC (US)
Assigned to Duke University, Durham, NC (US)
Filed by Duke University, Durham, NC (US)
Filed on Jan. 30, 2023, as Appl. No. 18/103,343.
Application 18/103,343 is a continuation of application No. 16/317,427, granted, now 11,596,683, previously published as PCT/US2017/041737, filed on Jul. 12, 2017.
Claims priority of provisional application 62/505,256, filed on May 12, 2017.
Claims priority of provisional application 62/361,131, filed on Jul. 12, 2016.
Prior Publication US 2023/0285537 A1, Sep. 14, 2023
This patent is subject to a terminal disclaimer.
Int. Cl. C07H 21/02 (2006.01); A61K 39/12 (2006.01); A61K 39/145 (2006.01); A61K 39/39 (2006.01); A61P 31/16 (2006.01); C07K 14/00 (2006.01); C07K 14/005 (2006.01); C07K 14/11 (2006.01); C12N 7/00 (2006.01); C12N 9/24 (2006.01); C12N 15/86 (2006.01); A61K 39/00 (2006.01)
CPC A61K 39/145 (2013.01) [A61K 39/12 (2013.01); A61K 39/39 (2013.01); A61P 31/16 (2018.01); C07K 14/00 (2013.01); C07K 14/005 (2013.01); C07K 14/11 (2013.01); C12N 7/00 (2013.01); C12N 9/2402 (2013.01); C12N 15/86 (2013.01); A61K 2039/5252 (2013.01); C07K 2319/42 (2013.01); C07K 2319/50 (2013.01); C07K 2319/60 (2013.01); C12N 2760/16121 (2013.01); C12N 2760/16122 (2013.01); C12N 2760/16134 (2013.01); C12N 2760/16143 (2013.01); C12Y 302/01018 (2013.01)] 15 Claims
OG exemplary drawing
 
1. A polynucleotide encoding a polypeptide comprising from N-terminus to C-terminus: a neuraminidase (NA) polypeptide, a furin site, a self-cleaving 2A polypeptide, and a hemagglutinin (HA) polypeptide, wherein both the NA polypeptide and the HA polypeptide that are expressed from the polynucleotide are free of residual amino acids from the furin site and the self-cleaving 2A polypeptide.