US 12,133,881 B2
Electrical stimulation of cells to induce enhanced secretome for therapeutic applications
Christine E. Schmidt, Gainesville, FL (US); and Sahba Mobini, Tres Cantos Madrid (ES)
Assigned to University of Florida Research Foundation, Inc., Gainesville, FL (US)
Appl. No. 17/263,263
Filed by University of Florida Research Foundation, Inc., Gainesville, FL (US)
PCT Filed Aug. 1, 2019, PCT No. PCT/US2019/044731
§ 371(c)(1), (2) Date Jan. 26, 2021,
PCT Pub. No. WO2020/028708, PCT Pub. Date Feb. 6, 2020.
Claims priority of provisional application 62/746,280, filed on Oct. 16, 2018.
Claims priority of provisional application 62/713,218, filed on Aug. 1, 2018.
Prior Publication US 2021/0187070 A1, Jun. 24, 2021
Int. Cl. A61K 38/18 (2006.01); A61K 9/00 (2006.01); A61K 9/06 (2006.01); A61K 35/28 (2015.01); A61K 35/32 (2015.01); A61K 35/34 (2015.01); A61K 35/44 (2015.01); C12N 5/071 (2010.01); C12N 5/077 (2010.01); C12N 5/0775 (2010.01); C12N 13/00 (2006.01)
CPC A61K 38/18 (2013.01) [A61K 9/0019 (2013.01); A61K 9/06 (2013.01); A61K 35/28 (2013.01); A61K 35/32 (2013.01); A61K 35/34 (2013.01); A61K 35/44 (2013.01); C12N 5/0655 (2013.01); C12N 5/0657 (2013.01); C12N 5/0667 (2013.01); C12N 5/069 (2013.01); C12N 13/00 (2013.01)] 7 Claims
 
1. A method of treating a human having a spinal cord injury, consisting of administering to the human in need thereof a composition consisting essentially of a secretome derived from electrically stimulated cells in a therapeutically effective amount to treat the spinal cord injury in the human in need thereof, wherein the cells have been electrically stimulated in an electrical field of from about 20 V/m to about 50 V/m.