	US 12,133,870 B2
	Treatment of opioid addiction using fibroblasts and products thereof
Pete O'Heeron, Houston, TX (US); and Thomas Ichim, San Diego, CA (US)
Assigned to SPINALCYTE LLC, Houston, TX (US)
Filed by SPINALCYTE LLC, Houston, TX (US)
Filed on Mar. 18, 2020, as Appl. No. 16/822,746.
Claims priority of provisional application 62/820,721, filed on Mar. 19, 2019.
Prior Publication US 2020/0297773 A1, Sep. 24, 2020
Int. Cl. A61K 35/33 (2015.01); C12N 5/077 (2010.01)

CPC A61K 35/33 (2013.01) [C12N 5/0656 (2013.01); C12N 2501/113 (2013.01); C12N 2501/115 (2013.01); C12N 2501/15 (2013.01)]

31 Claims

1. A method of treating addiction or substance abuse in a subject, the method comprising administering to the subject a composition comprising a fibroblast cell, a plurality thereof and/or a product derived thereof,

wherein the fibroblast cell is cultured in a growth medium, in a conditioned medium or in a reprogramming medium, and wherein the fibroblast cell expresses CD31 and/or CD73 markers,

wherein said fibroblast cell is generated by a method comprising the step of culturing under suitable conditions a plurality of fibroblasts, obtaining a single cell suspension from said plurality, and extracting from said single cell suspension fibroblast cells expressing markers CD31 and/or CD73, and

wherein culturing of said fibroblast cells occurs in a conditioned medium.