	US 12,133,864 B1
	Method for treating cancer cells with a Cu/Ag particle composition
Muhammad Nawaz, Dammam (SA); and Firdos Alam Khan, Dammam (SA)
Assigned to Imam Abdulrahman Bin Faisal University, Dammam (SA)
Filed by Imam Abdulrahman Bin Faisal University, Dammam (SA)
Filed on Jul. 9, 2024, as Appl. No. 18/766,716.
Application 18/766,716 is a continuation of application No. 18/599,582, filed on Mar. 8, 2024, granted, now 12,090,169.
Application 18/599,582 is a continuation of application No. 17/579,087, filed on Jan. 19, 2022, granted, now 11,980,638, issued on May 14, 2024.
This patent is subject to a terminal disclaimer.
Int. Cl. A61K 33/38 (2006.01); A61K 9/14 (2006.01); A61P 35/00 (2006.01); C01B 25/45 (2006.01); C01G 3/02 (2006.01)

CPC A61K 33/38 (2013.01) [A61K 9/14 (2013.01); A61P 35/00 (2018.01); C01B 25/45 (2013.01); C01G 3/02 (2013.01); C01P 2002/72 (2013.01); C01P 2002/82 (2013.01); C01P 2002/84 (2013.01); C01P 2002/85 (2013.01); C01P 2004/03 (2013.01); C01P 2004/32 (2013.01); C01P 2006/12 (2013.01); C01P 2006/14 (2013.01); C01P 2006/16 (2013.01)]

7 Claims

1. A method of decreasing cancer cell viability colorectal cancer cells in a cell culture, comprising:

contacting, in vitro, a Cu—Ag₃PO₄composition to the colorectal cancer cells and incubating in a CO₂incubator,

wherein the Cu—Ag₃PO₄composition comprises substantially spherical Cu—Ag₃PO₄nanoparticles having a mean particle size of 100-1,000 nm,

wherein an amount of the Cu—Ag₃PO₄nanoparticles is 5.0 to 95 g per mL of the cell culture,

wherein the Cu—Ag₃PO₄nanoparticles have an IC₅₀of 36-46 μg per mL of the cell culture,

wherein the colorectal cancer cells are HCT-116 cells,

wherein copper is present in the Cu—Ag₃PO₄nanoparticles in an amount of 10-15 weight percent (wt. %) based on the total weight of the Cu—Ag₃PO₄nanoparticles.