	US 12,133,863 B1
	Green synthesis of silver nanoparticles encapsulated in a nanostructured lipid carrier
Manal Ahmed Awad, Riyadh (SA); Khalid Mustafa Ortashi, Riyadh (SA); and Ali Kanakhr Aldalbahi, Riyadh (SA)
Assigned to KING SAUD UNIVERSITY, Riyadh (SA)
Filed by KING SAUD UNIVERSITY, Riyadh (SA)
Filed on Feb. 21, 2024, as Appl. No. 18/583,453.
Application 18/583,453 is a continuation of application No. 18/206,740, filed on Jun. 7, 2023.
Int. Cl. A61K 33/38 (2006.01); A61K 9/127 (2006.01); A61K 9/51 (2006.01); A61P 17/02 (2006.01); A61P 31/04 (2006.01); B82Y 5/00 (2011.01)

CPC A61K 33/38 (2013.01) [A61K 9/1271 (2013.01); A61K 9/1277 (2013.01); A61K 9/5176 (2013.01); A61P 17/02 (2018.01); A61P 31/04 (2018.01); B82Y 5/00 (2013.01)]

2 Claims

1. A method of preparing silver nanoparticles encapsulated in a nanostructured lipid carrier, the method consisting of:

providing a preparation of a sheep lipid in chloroform;

evaporating the chloroform from the preparation;

adding water to the preparation;

vortexing the preparation to completely disperse the sheep lipid in an aqueous phase;

dropwise adding the lipid in the aqueous phase to an aqueous solution of nanoparticles which have been prepared by a method consisting of:

adding Acacia nilotica to boiling water,

soaking the Acacia nilotica overnight, and filtering to obtain the Acacia nilotica extract;

adding the Acacia nilotica extract to an 0.1M aqueous solution of silver nitrate (AgNO₃) to obtain a mixture; and

stirring the mixture at a temperature of about 45° C. until a color of the mixture changes from colorless to brown, thereby indicating formation of the aqueous solution of silver nanoparticles

and

stirring the aqueous solution at about 20,000 rpm at a temperature of about 45° C. to obtain the silver nanoparticles encapsulated in the nanostructured lipid carrier.