US 12,133,728 B2
Systems and methods for evaluating tissue of a subject
John R. Chevillet, Seattle, WA (US); Tatiana D. Khokhlova, Seattle, WA (US); George R. Schade, Seattle, WA (US); Joo Ha Hwang, Bellevue, WA (US); and Muneesh Tewari, Seattle, WA (US)
Assigned to UNIVERSITY OF WASHINGTON, Seattle, WA (US); and FRED HUTCHINSON CANCER RESEARCH CENTER, Seattle, WA (US)
Filed by University of Washington, Seattle, WA (US); and Fred Hutchinson Cancer Research Center, Seattle, WA (US)
Filed on Jan. 6, 2023, as Appl. No. 18/094,206.
Application 18/094,206 is a continuation of application No. 16/936,095, filed on Jul. 22, 2020, abandoned.
Application 16/936,095 is a continuation of application No. 15/122,394, abandoned, previously published as PCT/US2015/024144, filed on Apr. 2, 2015.
Claims priority of provisional application 62/072,915, filed on Oct. 30, 2014.
Claims priority of provisional application 61/974,317, filed on Apr. 2, 2014.
Prior Publication US 2024/0041358 A1, Feb. 8, 2024
Int. Cl. A61B 5/145 (2006.01); A61B 5/00 (2006.01); A61B 5/15 (2006.01); A61B 8/08 (2006.01); A61B 10/00 (2006.01); A61N 7/02 (2006.01); C12Q 1/6886 (2018.01)
CPC A61B 5/14546 (2013.01) [A61B 5/145 (2013.01); A61B 5/14507 (2013.01); A61B 5/15 (2013.01); A61B 5/4836 (2013.01); A61B 8/08 (2013.01); A61B 8/5223 (2013.01); A61B 10/0038 (2013.01); A61B 10/007 (2013.01); A61N 7/022 (2013.01); C12Q 1/6886 (2013.01); A61B 2010/0077 (2013.01); A61N 7/02 (2013.01); C12Q 2600/118 (2013.01); C12Q 2600/158 (2013.01)] 18 Claims
OG exemplary drawing
 
1. A system for treating a human patient, the system comprising:
an ultrasound source configured to deliver high intensity focused ultrasound energy to a target tissue mass of the patient;
a function generator operably coupled to the ultrasound source, wherein the function generator initiates a pulsing protocol for delivering the high intensity focused ultrasound energy from the ultrasound source to the target tissue mass; and
a controller in communication with the ultrasound source and the function generator, wherein controller is configured to perform operations comprising—
applying high intensity focused ultrasound energy having a pulse average intensity of between 25 kW/cm2 and 60 k W/cm2 to generate shock waves that induce cavitation in the target tissue mass, the shock waves having a peak positive amplitude of between 78 MPa and 100 MPa, and wherein the cavitation induced by the shock waves causes a biomarker to be released from within cells of the patient;
comparing (a) a baseline concentration of the biomarker from a first fluid sample of the patient to (b) a concentration of the biomarker in a second fluid sample of the subject within 1 hour after applying high intensity focused ultrasound energy to the target tissue mass; and
repeating the applying and comparing until the concentration of the biomarker in the second fluid sample falls below a threshold value.