	US 12,133,528 B2
	Insecticidal proteins
Clarence Michael Reynolds, Research Triangle Park, NC (US); and Christopher Fleming, Research Triangle Park, NC (US)
Assigned to Syngenta Participations AG, Basel (CH)
Appl. No. 16/980,715
Filed by SYNGENTA PARTICIPATIONS AG, Basel (CH)
PCT Filed Mar. 7, 2019, PCT No. PCT/US2019/021093 § 371(c)(1), (2) Date Sep. 14, 2020, PCT Pub. No. WO2019/177855, PCT Pub. Date Sep. 19, 2019.
Claims priority of provisional application 62/643,275, filed on Mar. 15, 2018.
Prior Publication US 2021/0251240 A1, Aug. 19, 2021
Int. Cl. C12N 15/82 (2006.01); A01N 37/46 (2006.01); A01N 63/50 (2020.01); C07K 14/195 (2006.01)

CPC A01N 37/46 (2013.01) [A01N 63/50 (2020.01); C07K 14/195 (2013.01); C12N 15/8286 (2013.01)]

19 Claims

1. An expression cassette comprising a promoter operably linked to a heterologous nucleic acid molecule, said molecule comprising:

(a) the nucleotide sequence of SEQ ID NO: 9; or

(b) a nucleotide sequence that encodes a polypeptide, wherein the amino acid sequence of the polypeptide comprises SEQ ID NO: 47; or

(c) a nucleotide sequence that encodes a polypeptide, wherein the amino acid sequence of the polypeptide comprises SEQ ID NO: 39 having amino acid substitutions at positions Y213 and I215.