US 12,133,515 B2
Multiplexed genome editing
George M. Church, Brookline, MA (US); Luhan Yang, Somerville, MA (US); and Marc Guell, Barcelona (ES)
Assigned to President and Fellows of Harvard College, Cambridge, MA (US)
Filed by President and Fellows of Harvard College, Cambridge, MA (US)
Filed on Dec. 11, 2020, as Appl. No. 17/118,684.
Application 16/708,655 is a division of application No. 16/531,351, filed on Aug. 5, 2019, granted, now 11,064,684.
Application 17/118,684 is a continuation of application No. 16/708,655, filed on Dec. 10, 2019, granted, now 10,925,263.
Application 16/531,351 is a continuation of application No. 15/766,551, granted, now 10,959,413, previously published as PCT/US2016/055916, filed on Oct. 7, 2016.
Claims priority of provisional application 62/239,239, filed on Oct. 8, 2015.
Prior Publication US 2021/0100225 A1, Apr. 8, 2021
This patent is subject to a terminal disclaimer.
Int. Cl. C12N 15/85 (2006.01); A01K 67/0273 (2024.01); A01K 67/0275 (2024.01); A01K 67/0276 (2024.01); C12N 7/00 (2006.01); C12N 9/22 (2006.01); C12N 15/11 (2006.01); C12N 15/113 (2010.01); C12N 15/63 (2006.01); C12N 15/90 (2006.01); C07H 21/02 (2006.01); C07H 21/04 (2006.01)
CPC A01K 67/0275 (2013.01) [A01K 67/0273 (2013.01); A01K 67/0276 (2013.01); C12N 7/00 (2013.01); C12N 9/22 (2013.01); C12N 15/11 (2013.01); C12N 15/1132 (2013.01); C12N 15/63 (2013.01); C12N 15/85 (2013.01); C12N 15/8509 (2013.01); C12N 15/907 (2013.01); A01K 2207/05 (2013.01); A01K 2207/10 (2013.01); A01K 2227/108 (2013.01); A01K 2267/025 (2013.01); C07H 21/02 (2013.01); C07H 21/04 (2013.01); C12N 2310/20 (2017.05); C12N 2740/10062 (2013.01); C12N 2800/80 (2013.01)] 19 Claims
 
1. An isolated porcine organ comprising a plurality of genetically engineered porcine cells, wherein each genetically engineered porcine cell of said plurality is characterized by a plurality of endogenous retrovirus genes and in that at least 20% of the endogenous retrovirus genes comprise a genetically engineered genomic mutation.