US 12,133,512 B2
Artificial propagation method of Sinocyclocheilus rhinocerous
Xiao'ai Wang, Kunming (CN); Xiaofu Pan, Kunming (CN); Yuanwei Zhang, Kunming (CN); Junxing Yang, Kunming (CN); and Bolin Lu, Kunming (CN)
Assigned to Kunming Institute of Zoology, Chinese Academy of Sciences, Kunming (CN)
Filed by Kunming Institute of Zoology, Chinese Academy of Sciences, Kunming (CN)
Filed on Feb. 1, 2021, as Appl. No. 17/164,143.
Claims priority of application No. 202011410487.2 (CN), filed on Dec. 4, 2020.
Prior Publication US 2022/0175507 A1, Jun. 9, 2022
Int. Cl. A61D 19/02 (2006.01); A01K 61/10 (2017.01); A01K 61/17 (2017.01); A61L 2/00 (2006.01); A61L 101/02 (2006.01); C12N 5/073 (2010.01)
CPC A01K 61/10 (2017.01) [A01K 61/17 (2017.01); A61D 19/021 (2013.01); C12N 5/0604 (2013.01)] 18 Claims
 
1. An artificial propagation method of Sinocyclocheilus rhinocerous, comprising the following steps:
placing female and male parent fish in a sterilized culture container for parent fish culture, wherein black cave simulant is placed in the culture container in advance; environmental parameters during the parent fish culture are as follows: water temperature is in a range of 16-20° C., water flow velocity is in a range of 5-20 cm/s, concentration of dissolved oxygen of water is in a range of 6.0-7.0 mg/L, and water pH is in a range of 6.8-7.2, keeping the water clean; keeping a culture environment dark and noiseless;
during the parent fish culture, feeding pellet feed twice a day, and feeding amount is according to feed coefficient of 3%-4%, the pellet feed is a mixture of mash feed containing 40%-50% crude protein, earthworm, shrimp, vitamin D3 calcium tablets, multi-vitamin tablets and water, with a mass ratio of 250-280:80-100:10-20:10-20:8-20:300;
collecting sperm and eggs for artificial inseminating after gonads of female and male parent fish mature, rinsing sperm-egg mixture with normal saline with osmotic pressure of 280-320 mOsm/L and temperature of 18-20° C. for 3-4 times, then splashing the sperm-egg mixture on a sterilized palm sheet, and incubating the palm sheet adhered with fertilized eggs in an incubating pond for 7-8 days, breeding incubating fries to obtain adult fish of Sinocyclocheilus rhinocerous.