US 12,462,591 B2
Automated cell culture analysis and classification and detection
Steven Smith, Fitchburg, WI (US); and Takashi Wakui, Kanagawa (JP)
Assigned to FUJIFILM Cellular Dynamics, Inc., Madison, WI (US)
Filed by FUJIFILM Cellular Dynamics, Inc., Valhalla, NY (US)
Filed on Apr. 21, 2023, as Appl. No. 18/305,021.
Claims priority of provisional application 63/363,333, filed on Apr. 21, 2022.
Prior Publication US 2023/0343118 A1, Oct. 26, 2023
Int. Cl. G06V 20/69 (2022.01); G06T 7/00 (2017.01); G06T 7/11 (2017.01); G06V 10/25 (2022.01); G06V 10/44 (2022.01); G06V 10/54 (2022.01); G06V 10/56 (2022.01); G06V 10/776 (2022.01); G06V 10/98 (2022.01)
CPC G06V 20/698 (2022.01) [G06T 7/0012 (2013.01); G06T 7/11 (2017.01); G06V 10/25 (2022.01); G06V 10/54 (2022.01); G06V 10/776 (2022.01); G06V 10/98 (2022.01); G06V 20/695 (2022.01); G06T 2207/30024 (2013.01); G06T 2207/30108 (2013.01); G06V 10/44 (2022.01); G06V 10/56 (2022.01); G06V 2201/06 (2022.01)] 25 Claims
OG exemplary drawing
 
1. A computer-implemented method comprising:
obtaining, by one or more processors of the computing device, an image, wherein the image depicts a cell culture comprising more than one cell type;
applying, by the one or more processors, one or more nuclei detection algorithms to detect nuclear aspects in the image;
based on detecting the nuclear aspects, generating, by the one or more processors, a nuclear segmentation map;
utilizing, by the one or more processors, the nuclear segmentation map to identify one or more regions of interest in the image; and
generating a classification result by automatically determining, by the one or more processors, statistical information of the cells.