US 12,461,355 B2
Open-top light-sheet microscopy with a non-orthogonal arrangement of illumination and collection objectives
Adam K. Glaser, Seattle, WA (US); and Jonathan T. C. Liu, Seattle, WA (US)
Assigned to University of Washington, Seattle, WA (US)
Filed by University of Washington, Seattle, WA (US)
Filed on Mar. 23, 2023, as Appl. No. 18/189,018.
Application 18/189,018 is a continuation of application No. 17/737,736, filed on May 5, 2022, granted, now 11,644,656.
Application 18/189,018 is a continuation of application No. PCT/US2020/060530, filed on Nov. 13, 2020.
Claims priority of provisional application 62/934,758, filed on Nov. 13, 2019.
Prior Publication US 2023/0341669 A1, Oct. 26, 2023
This patent is subject to a terminal disclaimer.
Int. Cl. G02B 21/06 (2006.01); G02B 21/02 (2006.01); G02B 21/33 (2006.01); G02B 21/36 (2006.01)
CPC G02B 21/06 (2013.01) [G02B 21/02 (2013.01); G02B 21/33 (2013.01); G02B 21/361 (2013.01)] 36 Claims
OG exemplary drawing
 
12. An apparatus comprising:
a sample holder comprising a first surface and a second surface opposite the first surface, wherein the first surface is configured to support a sample;
an immersion chamber configured to hold an immersion fluid;
an illumination objective configured to direct an illumination light sheet towards the sample at an angle which is non-orthogonal to the first surface of the sample holder, the immersion chamber positioned between the illumination objective and the second surface of the sample holder such that the illumination objective directs the illumination light sheet through an ambient medium, through the immersion fluid, and through a material of the sample holder to a focal region of the sample; and
a collection objective configured to collect light along a collection axis which is approximately orthogonal to the first surface, the light having been having passed through the material of the sample holder and the immersion fluid.