US 12,461,108 B2
Mass spectrometry kit including a cross-linked antibody or fragment thereof
David L. Murray, Rochester, MN (US); Stephen Harding, Birmingham (GB); David R. Barnidge, Rochester, MN (US); Gregg Wallis, Birmingham (GB); John Mills, Rochester, MN (US); and Jamie Ashby, Birmingham West Midlands (GB)
Assigned to THE BINDING SITE GROUP LIMITED, Birmingham (GB); and MAYO FOUNDATION FOR MEDICAL EDUCATION AND RESEARCH, Rochester, MN (US)
Appl. No. 16/078,986
Filed by THE BINDING SITE GROUP LIMITED, Birmingham (GB); and MAYO FOUNDATION FOR MEDICAL EDUCATION AND RESEARCH, Rochester, MN (US)
PCT Filed Feb. 24, 2017, PCT No. PCT/GB2017/050489
§ 371(c)(1), (2) Date Aug. 22, 2018,
PCT Pub. No. WO2017/144900, PCT Pub. Date Aug. 31, 2017.
Claims priority of provisional application 62/368,606, filed on Jul. 29, 2016.
Claims priority of application No. 1603291 (GB), filed on Feb. 25, 2016.
Prior Publication US 2019/0094239 A1, Mar. 28, 2019
This patent is subject to a terminal disclaimer.
Int. Cl. G01N 33/68 (2006.01); C07K 16/00 (2006.01); C07K 16/42 (2006.01)
CPC G01N 33/6854 (2013.01) [C07K 16/00 (2013.01); C07K 16/42 (2013.01); G01N 33/6893 (2013.01); C07K 2317/54 (2013.01); G01N 2560/00 (2013.01); G01N 2800/24 (2013.01)] 19 Claims
 
1. A composition comprising a population of polyclonal anti-immunoglobulin specific antibodies or anti-immunoglobulin specific fragments thereof, wherein at least 70% of the antibodies or fragments thereof of the population comprise one or more non-reducible thioether containing cross-links between at least one heavy chain or fragment thereof and at least one light chain or fragment thereof of the antibodies or fragments thereof;
wherein said one or more non-reducible thioether containing cross-links replace one or more naturally occurring disulphide bonds between said at least one heavy chain or fragment thereof, and said at least one light chain or fragment thereof; and
further, wherein said non-reducible thioether containing cross-links are introduced by a reagent comprising a bis-maleimide cross-linker.