US 12,461,107 B2
Compositions, methods and systems for protein corona analysis and uses thereof
Xiaoyan Zhao, Foster City, CA (US); William Manning, Redwood City, CA (US); John Blume, Bellingham, WA (US); Lyndal Hesterberg, Loveland, CO (US); Gregory Troiano, Pembroke, MA (US); Michael Figa, San Mateo, CA (US); Hope Liou, Pleasanton, CA (US); and Shadi Roshdiferdosi, Belmont, CA (US)
Assigned to Seer, Inc., Redwood City, CA (US)
Filed by Seer, Inc., Redwood City, CA (US)
Filed on Aug. 31, 2022, as Appl. No. 17/823,924.
Application 17/823,924 is a continuation of application No. 17/216,510, filed on Mar. 29, 2021, abandoned.
Application 17/216,510 is a continuation of application No. PCT/US2020/024426, filed on Mar. 24, 2020.
Claims priority of provisional application 62/824,284, filed on Mar. 26, 2019.
Claims priority of provisional application 62/824,279, filed on Mar. 26, 2019.
Prior Publication US 2023/0076807 A1, Mar. 9, 2023
Int. Cl. G01N 33/68 (2006.01); G01N 33/543 (2006.01)
CPC G01N 33/6848 (2013.01) [G01N 33/54346 (2013.01)] 25 Claims
 
1. A method of serially interrogating a sample, the method comprising:
a) contacting the sample to a first particle type and incubating the first particle type with the sample to form a first biomolecule corona, wherein the first biomolecule corona forms upon incubation of the first particle type with the sample and wherein the first biomolecule corona comprises a protein;
b) separating the first particle type comprising the first biomolecule corona as a pellet from a supernatant of the sample;
c) contacting the supernatant of the sample to a second particle type and incubating the second particle type with the supernatant of the sample to form a second biomolecule corona, wherein the second biomolecule corona forms upon incubation of the second particle type with the supernatant of the sample, wherein the second biomolecule corona comprises a protein, and further wherein (i) the first particle type and the second particle type are a same particle type or (ii) the first particle type and the second particle type are different;
d) separating the second particle type comprising the second biomolecule corona from the supernatant of the sample; and
e) assaying the first biomolecule corona and the second biomolecule corona to determine a composition and concentration of a plurality of proteins in the sample based on the assayed first biomolecule corona and the assayed second biomolecule corona,
wherein the sample is serially interrogated with the first particle type before the second particle type.