	US 12,461,092 B2
	Screening method for APP cleavage activity-controlling substances of ADAMTS4
Naoki Kaneko, Kyoto (JP); Taisuke Tomita, Tokyo (JP); Yota Yoshizawa, Tokyo (JP); Masaya Matsuzaki, Tokyo (JP); and Miyabishara Yokoyama, Tokyo (JP)
Assigned to SHIMADZU CORPORATION, Kyoto (JP); and THE UNIVERSITY OF TOKYO, Tokyo (JP)
Appl. No. 17/606,289
Filed by SHIMADZU CORPORATION, Kyoto (JP); and THE UNIVERSITY OF TOKYO, Tokyo (JP)
PCT Filed Mar. 30, 2020, PCT No. PCT/JP2020/014433 § 371(c)(1), (2) Date Oct. 25, 2021, PCT Pub. No. WO2020/217865, PCT Pub. Date Oct. 29, 2020.
Claims priority of application No. 2019-086092 (JP), filed on Apr. 26, 2019.
Prior Publication US 2022/0326223 A1, Oct. 13, 2022
Int. Cl. G01N 33/50 (2006.01); C12Q 1/37 (2006.01)

CPC G01N 33/5038 (2013.01) [C12Q 1/37 (2013.01)]

8 Claims

1. A method for screening a compound that controls APP669 N-terminal cleavage activity of ADAMTS4, the method comprising:

contacting a compound with a cultured cell;

measuring an amount of at least one Aβ-related peptide produced from the cultured cell, wherein the at least one Aβ-related peptide comprises APP669-x, where x represents an integer larger than 669 and up to 770; and

evaluating the APP669 N-terminal cleavage activity of ADAMTS4 controlled by the compound in the cultured cell,

wherein in the evaluation of the APP669 N-terminal cleavage activity,

an amount of APP669-x in a control cell in which the compound is not contacted is used as a reference level, and

when an amount of APP669-x in a cell in which the compound is contacted is higher than the reference level, it is determined that the APP669 N-terminal cleavage activity is increased, and

when the amount of APP669-x in the cell on which the compound is contacted is lower than the reference level, it is determined that the APP669 N-terminal cleavage activity is decreased.