	US 12,461,090 B2
	Dimethylarsinous acid-induced malignantly transformed cell line of human keratinocytes and use thereof
Yan An, Suzhou (CN); Rui Yan, Suzhou (CN); Jiayuan Mao, Suzhou (CN); Qianlei Yang, Suzhou (CN); Haixuan Xia, Suzhou (CN); Qi Kong, Suzhou (CN); and Yuanjie Wei, Suzhou (CN)
Assigned to SOOCHOW UNIVERSITY, Suzhou (CN)
Appl. No. 17/625,740
Filed by SOOCHOW UNIVERSITY, Suzhou (CN)
PCT Filed Apr. 20, 2021, PCT No. PCT/CN2021/088303 § 371(c)(1), (2) Date Jan. 8, 2022, PCT Pub. No. WO2022/193398, PCT Pub. Date Sep. 22, 2022.
Claims priority of application No. 202110305323.1 (CN), filed on Mar. 19, 2021.
Prior Publication US 2023/0314407 A1, Oct. 5, 2023
Int. Cl. G01N 33/50 (2006.01); C12N 5/071 (2010.01); C12N 5/09 (2010.01)

CPC G01N 33/5011 (2013.01) [C12N 5/0629 (2013.01); C12N 5/0693 (2013.01); G01N 33/5044 (2013.01); C12N 2501/999 (2013.01); G01N 2500/10 (2013.01)]

7 Claims

1. A dimethylarsinous acid-induced malignantly transformed cell line of human keratinocytes, which is deposited in China General Microbiological Culture Collection Center (CGMCC, Address: Building #3, NO. 1 Beichen West Road, Chaoyang District, Beijing) under CGMCC Accession No. 21499 on Feb. 4, 2021,

wherein the dimethylarsinous acid-induced malignantly transformed cell line of human keratinocytes promotes an identification of carcinogenicity of arsenic methylated metabolites, and is obtained by a long-term exposure to 0.1 μM dimethylarsinous acid and adapted for indicating that a long-term exposure to low-dose arsenic metabolite dimethylarsinous acid causes a malignant transformation of skin cells.