| CPC G01N 30/88 (2013.01) [G01N 2030/027 (2013.01)] | 18 Claims |

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1. A kit for evaluating liquid chromatography (LC) system and column inertness, the kit comprising:
a first vial comprising a positive control comprising a metal interacting moiety, wherein the positive control is selected from the group consisting of 2′(3′)-O-(4-Benzoylbenzoyl) adenosine 5′- triphosphate (Bz-ATP), ATP labeled with 6-aminohexyladenosine, ATP labeled with methylanthraniloyl, ATP labeled with trinitrophenyl, ATP labeled with fluorescein, and ATP labeled with pyrene; and
a second vial comprising a negative control comprising caffeine, the negative control not containing a metal interacting moiety, wherein both the positive control and the negative control are used as probe compounds for the detection of exposed metal in LC system and column.
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