US 12,461,063 B2
Inorganic ion detection system and methods
Michael Charles Breadmore, Sandy Bay (AU); Philip James Charles Zakaria, Sandy Bay (AU); and Gustavo Blanco-Heras, A Coruña (ES)
Assigned to University of Tasmania, Tasmania (AU)
Appl. No. 16/322,471
Filed by University of Tasmania, Sandy Bay (AU)
PCT Filed Aug. 15, 2017, PCT No. PCT/AU2017/050861
§ 371(c)(1), (2) Date Jan. 31, 2019,
PCT Pub. No. WO2018/032043, PCT Pub. Date Feb. 22, 2018.
Claims priority of application No. 2016903232 (AU), filed on Aug. 15, 2016; and application No. 2017902304 (AU), filed on Jun. 16, 2017.
Prior Publication US 2019/0353614 A1, Nov. 21, 2019
Int. Cl. G01N 27/447 (2006.01); B01D 15/42 (2006.01); B01D 57/02 (2006.01); B01L 3/00 (2006.01); G01N 1/28 (2006.01); G01N 1/40 (2006.01); G01N 27/06 (2006.01)
CPC G01N 27/44739 (2013.01) [B01D 15/42 (2013.01); B01D 57/02 (2013.01); B01L 3/502 (2013.01); G01N 27/06 (2013.01); G01N 27/447 (2013.01); G01N 27/4473 (2013.01); G01N 27/44743 (2013.01); G01N 27/44791 (2013.01); B01L 2300/025 (2013.01); B01L 2300/0645 (2013.01); B01L 2300/16 (2013.01); G01N 2001/2833 (2013.01); G01N 1/405 (2013.01); G01N 2001/4061 (2013.01)] 10 Claims
OG exemplary drawing
 
1. A method for the separation and detection of nitrate, perchlorate, azide, chloride and chlorate anions in a sample using electrophoresis, the method comprising a sample processing sequence which comprises:
introducing a sample and a background electrolyte comprising polyethyleneimine into a fused silica capillary separation channel having an internal diameter of from 20 μm to 30 μm, coated with alternating layers of a cationic polymer and an anionic polymer, wherein a first layer and a final layer of the fused silica capillary separation channel coating are cationic polymer layers, wherein the alternating layers consist of two cationic polymer layers and one anionic polymer layer;
applying a voltage potential across the fused silica capillary separation channel to effect a separation of the nitrate, perchlorate, azide, chloride and chlorate anions, wherein the voltage potential provides a field strength of between 1.0 kV/cm and 3.3 kV/cm of capillary across which the voltage potential is applied; and
detecting the presence of the separated nitrate, perchlorate, azide and chlorate anions, wherein the cationic polymer forming the two cationic polymer layers is a hexadimethrine polymer, and the anionic polymer forming the anionic polymer layers is a polystyrenesulfonate polymer.