US 12,461,054 B2
Apparatus and methods for performing electrochemical reactions
John Nobile, Guilford, CT (US); George Thomas Roth, Fairfield, CT (US); Todd Rearick, Cheshire, CT (US); Jonathan Schultz, Oxford, MA (US); Jonathan M. Rothberg, Guilford, CT (US); and David Marran, Durham, CT (US)
Assigned to Life Technologies Corporation, Carlsbad, CA (US)
Filed by LIFE TECHNOLOGIES CORPORATION, Carlsbad, CA (US)
Filed on Dec. 8, 2022, as Appl. No. 18/077,404.
Application 18/077,404 is a continuation of application No. 14/162,612, filed on Jan. 23, 2014, granted, now 11,567,029.
Application 14/162,612 is a continuation of application No. 12/785,716, filed on May 24, 2010, granted, now 8,673,627, issued on Mar. 18, 2014.
Application 12/785,716 is a continuation in part of application No. 12/474,897, filed on May 29, 2009, abandoned.
Application 12/785,716 is a continuation in part of application No. 12/475,311, filed on May 29, 2009, abandoned.
Claims priority of provisional application 61/306,924, filed on Feb. 22, 2010.
Prior Publication US 2023/0101252 A1, Mar. 30, 2023
This patent is subject to a terminal disclaimer.
Int. Cl. G01N 27/30 (2006.01); B01L 3/00 (2006.01); G01N 27/414 (2006.01)
CPC G01N 27/301 (2013.01) [B01L 3/502761 (2013.01); G01N 27/4145 (2013.01); B01J 2219/00315 (2013.01); B01J 2219/00317 (2013.01); B01J 2219/005 (2013.01); B01J 2219/00653 (2013.01); B01J 2219/00722 (2013.01); B01L 2200/0668 (2013.01); B01L 2300/0636 (2013.01); B01L 2300/0645 (2013.01); B01L 2300/0877 (2013.01)] 20 Claims
OG exemplary drawing
 
11. A method of monitoring a reaction product in a reaction vessel, the method comprising:
flowing a first reagent within an apparatus, the apparatus comprising:
a reaction vessel coupled to an electronic sensor for monitoring a reaction product in the reaction vessel, wherein the reaction vessel includes a microwell array integrated with a sensor array;
a fluidics system for sequentially delivering a plurality of reagents to the reaction vessel, the fluidics system including a plurality of reagent reservoirs in fluidic communication via a plurality of flow paths with a fluidics circuit and to a common passage in fluidic communication between the fluidics circuit and the reaction vessel, a solution reservoir containing a solution in fluidic communication with the common passage via a branch passage connected with the common passage at a junction between the fluidics circuit and the reaction vessel, and a valve disposed between the solution reservoir and the junction; and
an electrode in contact with the solution within the branch passage, the electrode being disposed between the valve and the junction, the electrode being in electrical communication with the reaction vessel through fluid extending from the branch passage and through the common passage, wherein the electrode avoids direct contact with the plurality of reagents and uses the conductive solution for electrical contact, the electronic sensor generating an output signal depending on a voltage of the electrode;
flowing the solution through the branch channel and the common passage; and
flowing a second reagent within the apparatus.