US 12,461,030 B2
Point of care viral detection system using turbo fluorescence in situ hybridization
Brenna Singer, Gansevoort, NY (US); Bridget Bergstrom, Maple Grove, MN (US); Sarah M. Dunbar, Brookhaven, NY (US); Francheska Torres, Charlton, MA (US); Alana Persing, Allston, MA (US); Kelsey M. Dupont, Revere, MA (US); Timothy Lannin, Hopkinton, MA (US); and Jeffrey Ruberti, Lexington, MA (US)
Assigned to Northeastern University, Boston, MA (US)
Filed by Northeastern University, Boston, MA (US)
Filed on Mar. 10, 2022, as Appl. No. 17/692,078.
Claims priority of provisional application 63/159,423, filed on Mar. 10, 2021.
Prior Publication US 2022/0288586 A1, Sep. 15, 2022
Int. Cl. G01N 21/64 (2006.01); B01L 3/00 (2006.01); G01N 1/22 (2006.01); G01N 33/58 (2006.01)
CPC G01N 21/6428 (2013.01) [B01L 3/502715 (2013.01); B01L 3/502723 (2013.01); G01N 21/6454 (2013.01); G01N 33/582 (2013.01); B01L 2200/0684 (2013.01); B01L 2200/10 (2013.01); B01L 2200/16 (2013.01); B01L 2300/0663 (2013.01); B01L 2300/0803 (2013.01); B01L 2300/161 (2013.01); G01N 1/22 (2013.01); G01N 2001/2244 (2013.01); G01N 21/6402 (2013.01); G01N 2021/6463 (2013.01); G01N 2800/26 (2013.01)] 7 Claims
OG exemplary drawing
 
1. An analyte detection system comprising
(i) a device for detection of a fluorescently labeled analyte deposited on a solid detection surface of a disc the device comprising:
a rotator operative to continuously rotate the disc;
a laser operative to irradiate the analyte on the disc with excitation light capable of exciting a fluorophore bound to the analyte, wherein the laser is mounted on a scanning mechanism and irradiates a spot on the solid detection surface while the disc is rotated and scanned radially;
a detector coupled to the scanning mechanism and operative to detect fluorescence emission from the fluorophore during the scan; and
an analysis module that collects fluorescence emission data from the detector and stores, analyzes, and/or transmits the data to provide a measure of the analyte deposited on the solid detection surface; and
(ii) a device for fluorescently labeling an analyte disposed on a solid detection surface of a sample disc, the device comprising:
a circular disc holder capable of incremental, intermittent rotation around a central axis and having an outer edge an inner edge, and an empty center region, the disc holder comprising a plurality of incrementally spaced sample disc holders disposed between the outer edge and inner edge;
one or more stationary reagent dispensing stations disposed in the center region near the inner edge of the disc holder;
one or more cleaning vessels disposed within the center region; and
a control module;
wherein each dispensing station comprises two oppositely disposed dispensing/aspirating tubes capable of rotation to access either a sample disc in the disc holder or said one or more cleaning vessels;
wherein each dispensing station further comprises a rotation and dipping mechanism operative to place one of said dispensing/aspirating tubes above a sample disc for dispensing and/or aspirating a reagent to or from a solid detection surface of a sample disc or to place one of said dispensing/aspirating tube into said one or more cleaning vessels for cleaning; and
wherein the control module is configured to, according to a program, incrementally and intermittently rotate the circular disc holder, actuate selected reagent dispensing stations to dispense reagents onto the disc or aspirate reagents from the disc using said dispensing/aspirating tubes, and to deposit the dispensing/aspirating tubes into said one or more cleaning vessels.