US 12,461,009 B2
Method and microscope for recording trajectories of individual particles in a sample
Benjamin Harke, Gottingen (DE); Lars Kastrup, Gottingen (DE); and Christian Wurm, Gottingen (DE)
Assigned to ABBERIOR INSTRUMENTS GMBH, Gottingen (DE)
Appl. No. 18/265,308
Filed by ABBERIOR INSTRUMENTS GMBH, Gottingen (DE)
PCT Filed Dec. 21, 2021, PCT No. PCT/EP2021/086970
§ 371(c)(1), (2) Date Jun. 5, 2023,
PCT Pub. No. WO2022/136361, PCT Pub. Date Jun. 30, 2022.
Claims priority of application No. 10 2020 134 495.3 (DE), filed on Dec. 21, 2020.
Prior Publication US 2024/0035950 A1, Feb. 1, 2024
Int. Cl. G01N 15/14 (2024.01); G01N 15/1429 (2024.01); G01N 15/1434 (2024.01)
CPC G01N 15/1425 (2013.01) [G01N 15/1429 (2013.01); G01N 15/1434 (2013.01); G01N 15/1456 (2013.01)] 20 Claims
OG exemplary drawing
 
1. A method for recording a trajectory of an individual particle in a sample starting from an at least approximately known initial position of the particle, comprising the repeatedly executed method steps:
scanning the particle with an intensity distribution of a scanning light comprising a local minimum at one or more scanning positions;
detecting a number of photons or a light intensity emitted from the particle as a result of the illumination with the scanning light at each of the scanning positions as a first measured variable in a first detection channel;
determining updated coordinates of the particle from the photon number or light intensity detected at the scanning positions and adding the updated coordinates to the trajectory,
wherein during the recording of the trajectory a second measured variable is detected in the sample in a second detection channel and the recording of the trajectory is interrupted or terminated if the second measured variable or a control value calculated from the second measured variable fulfills a termination criterion, wherein the second measured variable is an optical measured variable detected in a vicinity around the current coordinates of the particle in the sample.